Open access · Peer reviewed · No APCs
Research ArticleAsian Journal of Biological and Life SciencesVol. 1 | Issue 2 | 2012 | pp. 79–82Open access
Cloning and Expression of Ketol-Acid Reductoisomerase (KARI) from Staphylococcus aureus
- 1,
- 1*
- 1 Department of Microbiology, Vivekanandha College of Arts and Sciences for Women [Autonomous], Elayampalayam, Tiruchengode, Namakkal-637205, Tamil Nadu, INDIA.
Published in Asian Journal of Biological and Life Sciences
Correspondence: P. Vijayalakshmi
Department of Microbiology, Vivekanandha College of Arts and Sciences for Women [Autonomous], Elayampalayam, Tiruchengode, Namakkal-637205, Tamil Nadu, INDIA.
Email: tprabu29@gmail.com
Copyright: © 2012 Manuscript Technomedia. This is an open access article.
- Published:
- Sep 10, 2012
- Received:
- Jul 13, 2012
- Accepted:
- Aug 3, 2012
How to cite
Rani, Y. S., & Vijayalakshmi, P. (2012). Cloning and Expression of Ketol-Acid Reductoisomerase (KARI) from Staphylococcus aureus. Asian Journal of Biological and Life Sciences, 1(2), 79–82.
Abstract
Ketol-acid reductoisomerase (EC 1.1.1.86) catalyzes the conversion of 2-aceto-2-hydroxyacids to 2-keto-3hydroxyacids and their subsequent reduction by NADPH to 2, 3-dihydroxyacids. The ilvC gene encoding the Staphylococcus aureus enzyme was cloned and expressed in E. coli carrying pRSET vector. The enzyme expression was tested based on the blue white screening of the transformed colonies. The work was carried out to enable the over expression of KARI and thereby can be used as a drug target.
Subject
Article metadata
| Title | Cloning and Expression of Ketol-Acid Reductoisomerase (KARI) from Staphylococcus aureus |
|---|---|
| Authors | Y. Sandhya Rani; P. Vijayalakshmi |
| Affiliations | Department of Microbiology, Vivekanandha College of Arts and Sciences for Women [Autonomous], Elayampalayam, Tiruchengode, Namakkal-637205, Tamil Nadu, INDIA. |
| Corresponding author | tprabu29@gmail.com |
| Journal | Asian Journal of Biological and Life Sciences |
| Volume / Issue | Vol. 1, Issue 2 (2012) |
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