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Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 47 | Issue 1 | 2013 | pp. 7–12Open access
Development and Validation of a new Reversed-phase HPLC Method for the Determination of Ezetimibe in Pharmaceutical Dosage forms
- 1,2*,
- 1,
- 2,
- 3
- 1 Ecron Acunova Manipal Acunova Clinical Research Centre, 4th Floor, Shirdi Sai Baba Cancer Hospital, Manipal - 576104, Karnataka.
- 2 Department of Pharmaceutical Chemistry Manipal University, Manipal. edu, Madhav Nagar, Manipal - 576104, Karnataka.
- 3 Genba Sopanrao Moze College of Pharmacy, Wagholi, Pune - 412207.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Ghanshyam Panjwani
Ecron Acunova Manipal Acunova Clinical Research Centre, 4th Floor, Shirdi Sai Baba Cancer Hospital, Manipal - 576104, Karnataka.; Department of Pharmaceutical Chemistry Manipal University, Manipal. edu, Madhav Nagar, Manipal - 576104, Karnataka.
Email: ghanshyampharma@gmail.com
Copyright: © 2013 Manuscript Technomedia. This is an open access article.
- Published:
- Mar 31, 2013
- Received:
- Mar 16, 2012
- Accepted:
- Jan 2, 2013
How to cite
Panjwani, G., Shetty, R., Shenoy, G., & Dhamecha, D. (2013). Development and Validation of a new Reversed-phase HPLC Method for the Determination of Ezetimibe in Pharmaceutical Dosage forms. Indian Journal of Pharmaceutical Education and Research, 47(1), 7–12.
Abstract
Ezetimibe belongs to a group of selective and very effective 2-azetidione cholesterol absorption inhibitors that act on the level of cholesterol entry into enterocytes. A new, rapid, specific reversed-phase HPLC method has been developed for assaying ezetimibe in pharmaceutical dosage forms. The assay involved an isocratic elution of ezetimibe in a Betasil C (250 mm x 4.6 mm i.d., 5μ) column using a mobile phase composition of 18 Acetonitrile: 10 mM Na HPO pH 7.0 (55:45 v/v). The flow rate was 1ml/min and the analyte monitored at 233nm. The assay method was found to 2 4 be linear from 4-24μg/mL. All the validation parameters were within the acceptance range. The developed method was successfully applied to estimate the amount of ezetimibe in tablets.
Keywords
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Article metadata
| Title | Development and Validation of a new Reversed-phase HPLC Method for the Determination of Ezetimibe in Pharmaceutical Dosage forms |
|---|---|
| Authors | Ghanshyam Panjwani; Ragvendra Shetty; Gautham Shenoy; Dinesh Dhamecha |
| Affiliations | Ecron Acunova Manipal Acunova Clinical Research Centre, 4th Floor, Shirdi Sai Baba Cancer Hospital, Manipal - 576104, Karnataka.; Department of Pharmaceutical Chemistry Manipal University, Manipal. edu, Madhav Nagar, Manipal - 576104, Karnataka.; Genba Sopanrao Moze College of Pharmacy, Wagholi, Pune - 412207. |
| Corresponding author | ghanshyampharma@gmail.com |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 47, Issue 1 (2013) |
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