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Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 50 | Issue 3 | 2026 | pp. 482–488Open access
Application of TLC-Densitometry for Analysis of Estradiol Hemihydrate in Dosage Forms
- 1,
- 1,2,
- 1,2,
- 3,
- 4
- 1 Medical University-Sofia, Faculty of Pharmacy, Department of Pharmaceutical Chemistry.
- 2 Medical University-Plovdiv, Faculty of Pharmacy, Department of Pharmacognosy and Pharmaceutical Chemistry, 15A Vasil Aprilov Str., Plovdiv 4002, BULGARIA.
- 3 Medical University-Plovdiv, Medical College, 15A Vasil Aprilov Str., Plovdiv 4002, BULGARIA.
- 4 Clinic of Internal Diseases UMHATEM ”N. I. Pirogov”-SOFIA, BULGARIA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Email: dobrinka30@abv.bg
Copyright: © 2026 Manuscript Technomedia. This is an open access article.
- Published:
- Aug 12, 2026
- Received:
- Apr 23, 2016
- Accepted:
- Jun 23, 2016
How to cite
Tsvetkova, D. D., Obreshkova, D. P., Ivanova, S. A., Hadjieva, B., & Atanasov, P. Y. (2026). Application of TLC-Densitometry for Analysis of Estradiol Hemihydrate in Dosage Forms. Indian Journal of Pharmaceutical Education and Research, 50(3), 482–488. https://doi.org/10.5530/ijper.50.3.23
Abstract
The aim of current study was the application of validated TLC-densitometric method for identification and determination of Estradiol hemihydrate in dosage forms. The applied TLC conditions were: Silicagel G60 F254 glass plates; mobile phase: chloroform : acetone = 90 : 10 v/v, migration distance of mobile phase: 120 mm, UV-detection at = 254 nm. All of the experimental results for the content of Estradiol hemihydrate correspond to the respective confidence interval: Estrofem table: 1.78 mg ÷ 2.12 mg; Femoston F1 table: 1.88 mg ÷ 2.2 mg; Femoston F2 table: 1.99 mg ÷ 2.19 mg; Trisequens T1 table: 1.78 mg ÷ 2.18 mg; Trisequens T2 table: 1.92 mg ÷ 2.12 mg; Trisequens T3 table: 0.97 mg ÷ 1.17 mg. The proposed validated TLC-densitometric method is appropriate for quality control of Estradiol hemihydrate in commercially available tablets.
Keywords
Subject
Article metadata
| Title | Application of TLC-Densitometry for Analysis of Estradiol Hemihydrate in Dosage Forms |
|---|---|
| Authors | Dobrina Doncheva Tsvetkova; Danka Petrova Obreshkova; Stefka Achkova Ivanova; Bozhidarka Hadjieva; Peter Yordanov Atanasov |
| Affiliations | Medical University-Sofia, Faculty of Pharmacy, Department of Pharmaceutical Chemistry.; Medical University-Plovdiv, Faculty of Pharmacy, Department of Pharmacognosy and Pharmaceutical Chemistry, 15A Vasil Aprilov Str., Plovdiv 4002, BULGARIA.; Medical University-Plovdiv, Medical College, 15A Vasil Aprilov Str., Plovdiv 4002, BULGARIA.; Clinic of Internal Diseases UMHATEM ”N. I. Pirogov”-SOFIA, BULGARIA. |
| Corresponding author | dobrinka30@abv.bg |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 50, Issue 3 (2026) |
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