Synthesis and Evaluation of Anticancer Activity of 1, 3, 4-Oxadiazole Derivatives against Ehrlich Ascites Carcinoma Bearing Mice and Their Correlation with Histopathology of Liver

A series of 2, 5-disubstituted 1, 3, 4-Oxadiazole derivatives (4A-4G) have been synthesized with the help of different aromatic benzaldehyde and final compounds were characterized by FT-IR, 1H NMR and Mass spectroscopy. The anticancer study was investigated against Ehrlich Ascites Carcinoma (EAC) bearing albino mice. The synthesized (4A-4G) compounds were administered intraperitoneally at dose of 20-25 mg/kg; body weight per day for 7 days after 24 hour of tumor inoculation in mice. The standard compound used was 5-FU (20 mg/kg; body, weight). Synthesized compounds (4A-4G) remarkably decreased the body weight, tumor volume, packed cell volume, viable cell count and increased in tumor weight (%) inhibition, tumor cells (%) inhibition, the life span, nonviable cell count of EAC tumor bearing mice when compared with the control group. The liver section of EAC treated control group (II) was compared with the drug treated groups (III-X). The histopathological observations of test groups suggested that normal architecture of liver nucleus, parenchyma, and hepatic cells were regenerated, which was damaged in EAC control group. All the synthesized compounds (4A-4G) showed significant anticancer activity in EAC bearing mice which encourages us to develop/improve similar other compounds and test them for their anticancer activity.