Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 53 | Issue 2 | 2019 | pp. 310–315Open access
Method Development and Validation for the Estimation of Dothiepin Hydrochloride by using RP-HPLC in PURE and Tablet Dosage Form
- 1*,
- 2,
- 3,
- 3,
- 3,
- 3
- 1 Department of Pharmaceutical Analysis, Associate Professor, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District.
- 2 Department of Pharmaceutical Analysis, Professor and Principal, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna.
- 3 Student, V.V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Prasanthi Thayi
Department of Pharmaceutical Analysis, Associate Professor, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District.
Email: prasanthi8585@gmail.com
Copyright: © 2019 Manuscript Technomedia. This is an open access article.
- Published:
- Feb 18, 2019
- Received:
- Jul 17, 2018
- Accepted:
- Jan 18, 2019
How to cite
Thayi, P., Atmakuri, L. R., Mandada, N., Mandava, H., Mandru, B., & Manne, C. (2019). Method Development and Validation for the Estimation of Dothiepin Hydrochloride by using RP-HPLC in PURE and Tablet Dosage Form. Indian Journal of Pharmaceutical Education and Research, 53(2), 310–315. https://doi.org/10.5530/ijper.53.2.39
Abstract
Aim: A simple, sensitive, accurate and precise RP-HPLC method was developed for the determination of Dothiepin HCl (DTH) in pure and tablet dosage form. Methods: The method was developed by using Phenomenex C18 (250 X 4.6 mm, 5 µm) and the mobile phase composed of buffer (0.1M sodium acetate): acetonitrile in the ratio of 50:50 v/v. The buffer pH was adjusted to 2.8. The retention time for Dothiepin HCl was found to be 3.44 min. Linearity range for Dothiepin HCl was found to be 10-60 µg/mL and the regression equation was found to be y=14691x-12844. % RSD for intra- and inter-day precision was found to be 0.27% and 0.84%. Average mean recovery was found to be 99.94%. LOD and LOQ values obtained for Dothiepin HCl were found to be 0.825 µg/mL and 2.498 µg/mL respectively. Conclusion: The results are analysed statistically and are found to be satisfactory. Hence this method can be successfully employed for analysis of Dothiepin HCl in tablet dosage form.
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Article metadata
| Title | Method Development and Validation for the Estimation of Dothiepin Hydrochloride by using RP-HPLC in PURE and Tablet Dosage Form |
|---|---|
| Authors | Prasanthi Thayi; Lakshman Rao Atmakuri; Nandini Mandada; Hemanth Mandava; Bhuvaneswari Mandru; Chaitanya Manne |
| Affiliations | Department of Pharmaceutical Analysis, Associate Professor, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District.; Department of Pharmaceutical Analysis, Professor and Principal, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna.; Student, V.V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA. |
| Corresponding author | prasanthi8585@gmail.com |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 53, Issue 2 (2019) |
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