Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 54 | Issue 2s | 2020 | pp. s295–s300Open access
In-vitro Cytotoxicity and in silico Molecular Docking of Alkaloids from Tiliacora acuminata
- 1,
- 1*,
- 1,
- 2
- 1 Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.
- 2 Department of Pharmacology and Toxicology, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Kirankumar Hullatti
Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.
Email: kkhullatti@gmail.com
Copyright: © 2020 Manuscript Technomedia. This is an open access article.
- Published:
- May 5, 2020
- Received:
- Nov 14, 2019
- Accepted:
- Mar 12, 2020
How to cite
Rodrigues, J., Hullatti, K., Jalalpure, S., & Khanal, P. (2020). In-vitro Cytotoxicity and in silico Molecular Docking of Alkaloids from Tiliacora acuminata. Indian Journal of Pharmaceutical Education and Research, 54(2s), s295–s300. https://doi.org/10.5530/ijper.54.2s.86
Abstract
Background: The present study aimed to identify the cytotoxicity of Tiliacora acuminata extract/fraction(s) plant via bioactivity guided approach and predict the binding affinity with Topoisomerase II. Materials and Methods: Extract and fractions were screened using brine shrimp lethality bioassay and the potent fraction was further evaluated for its in vitro cytotoxicity using five different cell lines i.e. HT-29, HepG2, MCF-7 and A-549. The binding affinity of individual phytoconstituent from the potent fraction with Topoisomerase II was further predicted using autodock4. Results: The fraction containing alkaloids showed the highest cytotoxicity against the HT-29 cell line. Molecular docking study identified coclaurine as a potent alkaloid phytoconstituent to bind with topoisomerase II. Conclusion: The study revealed the potential cytotoxic alkaloids from T. acuminata which may find use in the development of cytotoxic or chemopreventive agents.
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Article metadata
| Title | In-vitro Cytotoxicity and in silico Molecular Docking of Alkaloids from Tiliacora acuminata |
|---|---|
| Authors | Jeswiny Rodrigues; Kirankumar Hullatti; Sunil Jalalpure; Pukar Khanal |
| Affiliations | Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.; Department of Pharmacology and Toxicology, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA. |
| Corresponding author | kkhullatti@gmail.com |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 54, Issue 2s (2020) |
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