Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 59 | Issue 1 | 2024 | pp. 94–100Open access
Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage
- *
- 1 Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad, Kalaburagi, Karnataka, INDIA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Basavaraj Hiremath
Email: drbhiremath25@gmail.com
Copyright: © 2024 Manuscript Technomedia. This is an open access article.
- Published:
- Dec 20, 2024
- Received:
- Oct 30, 2023
- Accepted:
- Oct 19, 2024
How to cite
Hiremath, B. (2024). Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage. Indian Journal of Pharmaceutical Education and Research, 59(1), 94–100. https://doi.org/10.5530/ijper.20255575
Abstract
Aim
A sensitive and accurate RP-HPLC method was developed and validated for the quantitative measurement of Ceftriaxone sodium (CEF) in pharmaceutical dosage forms and bulk medicine.
Materials and Methods
Ceftriaxone sodium was separated on a 245 nm photodiode array detector using a Waters XTerra RP-18 (5 μm 250x4.6 mm internal diameter) column.
Results
The method generated an excellent linear response in the concentration range of 0.2-20 μg/mL with remarkable precision of 0.16-0.7% and accuracy in the percent recovery range of 99.88-99.97%.
Conclusion
We find the reverse phase HPLC technique to be highly sensitive, accurate, precise and user-friendly and hence recommend it. Therefore, it could be useful in a quality control lab.
Keywords
Subject
Article metadata
| Title | Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage |
|---|---|
| Authors | Basavaraj Hiremath |
| Affiliations | Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad, Kalaburagi, Karnataka, INDIA. |
| Corresponding author | drbhiremath25@gmail.com |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 59, Issue 1 (2024) |
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