Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 59 | Issue 2s | 2025 | pp. s740–s747Open access
Development and Validation of a HPLC-PDA Method to Quantification of Ketobemidone in Rat Plasma and its’ Application in Pharmacokinetic Study
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- 2*,
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- 1 Department of Chemistry, School of Applied Science and Humanities, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur, Andhra Pradesh, India.
- 2 Discovery Analytical R and D Department, Aragen Life Sciences Pvt. Ltd., IDA Nacharam, Hyderabad, Telangana, INDIA.
- 3 Polymer Engineering Laboratory, Department of Chemistry, PSG Institute of Technology and Applied Research, Neelambur, Coimbatore, Tamil Nadu, INDIA.
- 4 Department of Freshmen Engineering, St. Martin’s Engineering College, Dhulapally, Hyderabad, Telangana, INDIA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Shashipal Aitha
Department of Chemistry, School of Applied Science and Humanities, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur, Andhra Pradesh, India.
Email: subudeva@gmail.com
Copyright: © 2025 Manuscript Technomedia. This is an open access article.
- Published:
- Apr 23, 2025
- Received:
- May 16, 2024
- Accepted:
- Jan 2, 2025
How to cite
Aitha, S., Devaraju, S., Murthy, J. N. S. R. C., Tatapudi, K. K., Geddada, L. P., Somasekhar, T., Sadasivuni, H., & Kaliyaperumal, M. (2025). Development and Validation of a HPLC-PDA Method to Quantification of Ketobemidone in Rat Plasma and its’ Application in Pharmacokinetic Study. Indian Journal of Pharmaceutical Education and Research, 59(2s), s740–s747. https://doi.org/10.5530/ijper.20256924
Abstract
Aim:
Ketobemidone is an opioid drug that have been using against pain in various circumstances. The current research was aimed to develop and validate a HPLC-PDA method to estimate the ketobemidone in rat plasma.
Materials and Methods:
The ketobemidone in the biological matrices (rat plasma) was separated using solvent extraction method using methanol as extracting solvent. An isocratic mobile phase consisting of methanol-water-phosphate buffer at 40:60:0.01 % v/v/v and Kromasil C18 column were used for separation in Liquid Chromatography (LC).
Results:
The selected linearity range i.e., 0.10-25μg/mL was acceptable with r2 value 0.9998 as least square method. The determined Lower Limit of Quantification (LLOQ) is 0.10 μg/mL. The intermediate precision studies were performed within and between days; and the data obtained are within a significant range, i.e., %RSD <9.2 and the accuracy was within 10.0% of the relative error.
Conclusion:
The developed method to detect the ketobemidone was successfully validated and it was successfully applied on rat pharmacokinetic study.
Keywords
Subject
Article metadata
| Title | Development and Validation of a HPLC-PDA Method to Quantification of Ketobemidone in Rat Plasma and its’ Application in Pharmacokinetic Study |
|---|---|
| Authors | Shashipal Aitha; Subramani Devaraju; Jayanti Naga Sri Rama Chandra Murthy; Kiran Kumar Tatapudi; Leela Prasad Geddada; Tiruveedhula Somasekhar; Hemambika Sadasivuni; Muralidharan Kaliyaperumal |
| Affiliations | Department of Chemistry, School of Applied Science and Humanities, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur, Andhra Pradesh, India.; Discovery Analytical R and D Department, Aragen Life Sciences Pvt. Ltd., IDA Nacharam, Hyderabad, Telangana, INDIA.; Polymer Engineering Laboratory, Department of Chemistry, PSG Institute of Technology and Applied Research, Neelambur, Coimbatore, Tamil Nadu, INDIA.; Department of Freshmen Engineering, St. Martin’s Engineering College, Dhulapally, Hyderabad, Telangana, INDIA. |
| Corresponding author | subudeva@gmail.com |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 59, Issue 2s (2025) |
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