Film Forming, Antimicrobial and Growth Promoting Wound Healing Spray Formulation

Background: Bacterial infections at wound site are one of the major causes for delayed healing. The aim of this study was to develop topical film forming spray having Combination of Simvastatin and Mupirocin which can avoid such infections and provide enhanced wound healing. Methods: Film forming spray formulation having Simvastatin and Mupirocin was formulated using Eudragit E100as film forming agent and Glycerol and Polyethylene glycol (PEG-400) as plasticizers. The effect of these excipients on the formulation was assessed by applying factorial design. Optimized formulation was evaluated for swelling index, spray angle, spray pattern, in- vitro drug release, evaporation time, average weight per dose, antimicrobial efficacy and in-vivo wound healing was assessed and compared with marketed formulation by excision wound healing model. Results: The optimized batch of topical film forming spray having combination of Simvastatin and Mupirocin was selected based on the results of swelling index and in-vitro drug release at respective drugs. Both the drugs gave more than 80% of drug release within 2 hrs and showed satisfactory physicochemical and mechanical properties along with potent antimicrobial efficacy against S. aureus and E. coli. Developed formulation showed comparative wound healing activity as that of marketed conventional formulation. Conclusion: Topical film forming spray was successfully developed using combination of Simvastatin and Mupirocin Calcium. Antimicrobial activity and results of animal studies indicated safety and efficacy of formulation.