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Original ArticleInternational Journal of Pharmaceutical InvestigationVol. 13 | Issue 2 | 2023 | pp. 313–320Open access
Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone]
- 1*,
- 2,
- 3,
- 4,
- 1
- 1 Department of Pharmacology, NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Mangalore, Karnataka, INDIA.
- 2 Department of Pharmaceutical Chemistry, Yenepoya University (Deemed to be University), Yenepoya Pharmacy College and Research Center, Mangalore, Karnataka, INDIA.
- 3 Department of Advance Research Centre, (NGSMIPS), NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences Mangalore, Karnataka, INDIA.
- 4 Department of Pharmaceutical Chemistry, NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Mangalore, Karnataka, INDIA.
Published in International Journal of Pharmaceutical Investigation
Correspondence: Harsha Ashtekar
Department of Pharmacology, NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Mangalore, Karnataka, INDIA.
Email: hrshshtkr2@gmail.com
Copyright: © 2023 Manuscript Technomedia. This is an open access article.
- Published:
- Mar 3, 2023
- Received:
- Nov 18, 2022
- Accepted:
- Jan 30, 2023
How to cite
Ashtekar, H., Aggarwal, N., Raviraj, C., Rajesh, G. D., & Varghese, N. (2023). Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone]. International Journal of Pharmaceutical Investigation, 13(2), 313–320. https://doi.org/10.5530/ijpi.13.2.041
Abstract
Aim: A new simple, accurate, precise, Reverse Phase High-performance Liquid Chromatography method was developed and validated for the estimation of newly synthesized (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] derivative of pyrazolone. Materials and Methods: The chromatogram was run through Luna 5µ C18 (2). 250 × 4.80 mm. 272817-7 HPLC column; mobile phase consisting of acetonitrile and water in the ratio 90:10 was pumped through the column at a flow rate of 0.8mL/min. Results: The optimized wavelength was 237 nm. The retention time of (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] was found to be 7.3 min. The percentage relative standard deviation was obtained as 0.3%. Conclusion: This method was accurate, precise, and sensitive; therefore, could be used for the quality control and bioanalytical evaluation of (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] in drug testing laboratory.
Keywords
Subject
Article metadata
| Title | Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone] |
|---|---|
| Authors | Harsha Ashtekar; Natasha Aggarwal; Chaitra Raviraj; Gupta Dheeraj Rajesh; Nimmy Varghese |
| Affiliations | Department of Pharmacology, NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Mangalore, Karnataka, INDIA.; Department of Pharmaceutical Chemistry, Yenepoya University (Deemed to be University), Yenepoya Pharmacy College and Research Center, Mangalore, Karnataka, INDIA.; Department of Advance Research Centre, (NGSMIPS), NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences Mangalore, Karnataka, INDIA.; Department of Pharmaceutical Chemistry, NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Mangalore, Karnataka, INDIA. |
| Corresponding author | hrshshtkr2@gmail.com |
| Journal | International Journal of Pharmaceutical Investigation |
| Volume / Issue | Vol. 13, Issue 2 (2023) |
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