Simultaneous Estimation of Gallic Acid, Vasicine, Pterostilbene and Piperine Using RP-HPLC Method from Ayurvedic Proprietary Formulations

Introduction: A simple, accurate, precise, sensitive, and validated RP-HPLC method was developed for simultaneous estimation of Gallic Acid (GA), Vasicine (VS), Pterostilbene (PTS) and Piperine (PP) from Ayurvedic Proprietary Formulations developed in two different dosage forms viz. Amarantha Gynorite syrup and Amarantha Gynorite capsule. Materials and Methods: The optimized chromatographic condition was used for separation on Agilent Zorbax SB-C18 (5 µm 4.6 x 250 mm), Rheodyne injector with volume of 20 µL and mobile phase consisting of Solvent A (70 percent methanol) and solvent B [10 nm Hexane sulfonic acid, Acetonitrile and Glacial Acetic acid (70:30:1)] in composition of 95: 05 v/v with isocratic elution for 40 min at flow rate of 0.5 mL/ min. Quantification was carried out using a photodiode array detector at 280 nm. The method was validated in accordance with International Conference on Harmonization (ICH) guidelines. Results and Conclusion: No chromatographic interference from syrup and capsule excipients was analyzed. Hence, the present analytical method is applicable for simultaneous determination of GA, VS, PTS and PP in Ayurvedic proprietary formulations.