Optimization of Lipase Production from Pseudomonas fluorescens

Objectives: Lipases are widely used in biodiesel, food, detergent, and pharmaceutical industries. To fulfil the rising demand for these crucial industrial enzymes, an extensive study on lipases from various micro-organisms will be useful in maximising the growing conditions and reducing production costs. Materials and Methods: The current work concentrated on optimising the carbon source, inducer concentration, and growth parameters to produce lipases at a reasonable price from pseudomonas species. Results: Extracellular lipases from Pseudomonas fluorescens were produced and optimised, achieving a concentration of 220 IU/mL of submerged fermentation broth. Conclusion: The results showed that the extracellular lipase production from Pseudomonas fluorescens was enhanced by three folds using submerged fermentation.