Open access · Peer reviewed · No APCs
Short CommunicationJournal of Young PharmacistsVol. 11 | Issue 1 | 2019 | pp. 101–104Open access
Ex vivo and in silico Molecular Docking Studies of Aldose Reductase Inhibitory Activity of Apigenin from Morus indica L.
- 1,
- 2,
- 3,
- 1*
- 1 Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangotri, Mysuru -570 006, Karnataka, INDIA.
- 2 Department of Studies in Botany, University of Mysore, Manasagangotri, Mysuru -570 006, Karnataka, INDIA.
- 3 Department of Physics, The National Institute of Engineering (NIE), Mysuru – 570 008, Karnataka, INDIA.
Published in Journal of Young Pharmacists
Correspondence: Asna Urooj
Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangotri, Mysuru -570 006, Karnataka, INDIA.
Email: asnaurooj@foodsci.uni-mysore.ac.in
Copyright: © 2019 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 5, 2019
- Received:
- Jul 28, 2018
- Accepted:
- Oct 25, 2018
How to cite
Anandan, S., Mahadevamurthy, M., C, C., & Urooj, A. (2019). Ex vivo and in silico Molecular Docking Studies of Aldose Reductase Inhibitory Activity of Apigenin from Morus indica L. Journal of Young Pharmacists, 11(1), 101–104. https://doi.org/10.5530/jyp.2019.11.21
Abstract
Objective: An investigation on Aldose Reductase Enzyme (ALR) inhibitory activity of apigenin (API) isolated from Morus indica L. was evaluated by ex vivo and molecular docking studies. Materials and Methods: The inhibitory efficacy of API from M. indica was evaluated against ALR in lens tissue of mice by ex vivo and their binding mechanism through molecular docking was carried out by AutoDock. Results: The API (10-50 µg mL-1) concentration exhibited significant inhibition (p ≤ 0.05) of ALR enzyme in a dose dependent manner with IC50 value of 39.23 µg mL-1. The positive control aminoguanidine (AG) at 10 mM inhibited 42.96% of ALR. The molecular docking studies revealed that API showed better binding energy (-9.15 kJ mol-1) when compared to AG (-3.78 kJ mol1 ). Molecular interaction analysis showed that API interrupts the proton donation mechanism which is necessary for the catalytic activity of ALR by forming H-bond with Trp20 (proton donor). Conclusion: The ALR inhibition potential offered by API was further confirmed through molecular docking studies. The present findings support the pharmacological application of API for the treatment of diabetes cataract.
Keywords
Subject
Article metadata
| Title | Ex vivo and in silico Molecular Docking Studies of Aldose Reductase Inhibitory Activity of Apigenin from Morus indica L. |
|---|---|
| Authors | Satish Anandan; Murali Mahadevamurthy; Chandra C; Asna Urooj |
| Affiliations | Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangotri, Mysuru -570 006, Karnataka, INDIA.; Department of Studies in Botany, University of Mysore, Manasagangotri, Mysuru -570 006, Karnataka, INDIA.; Department of Physics, The National Institute of Engineering (NIE), Mysuru – 570 008, Karnataka, INDIA. |
| Corresponding author | asnaurooj@foodsci.uni-mysore.ac.in |
| Journal | Journal of Young Pharmacists |
| Volume / Issue | Vol. 11, Issue 1 (2019) |
Also in this issue
- Fast Dissolving Oral Film of Piroxicam: Solubility Enhancement by forming an Inclusion Complex with β-cyclodextrin, Formulation and Evaluationpp. 1–6
- Eudragit S-100 Encapsulated Chitosan Coated Liposomes Containing Prednisolone for Colon Targeting: In vitro, Ex vivo and In vivo Evaluationpp. 7–11
- Comparison of Bulk and Precipitation Polymerization Method of Synthesis Molecular Imprinted Solid Phase Extraction for Atenolol using Methacrylic Acidpp. 12–16
- Evaluation of Silver Nanoparticles Addition in Periodontal Dressing for Wound Tissue Healing by 99mTc-ciprofloxacinpp. 17–20
- Development and Validation of an RP-HPLC Method for Determination of Solasodine, a Steroidal Alkaloidpp. 21–25
Readers Also Viewed
Effect of the Fractions of Coccinia grandis on Ethanol- Induced Cerebral Oxidative Stress in Rats
M. Umamaheswari, T. K. Chatterjee
Jan 1, 2009
Phytochemical Screening and Antioxidant Activity Evaluation of Selected Philippine Fruit Peels and Pulps
Mildred Dagcuta Pates, Angelo Mark Po Walag, Romeo M. Del Rosario
Aug 17, 2026
Impact of Artificial Intelligence Assisted Drug Therapy Optimization on Drug Related Problems Amongst Older Adult Patients: A Systematic Review
Jehath Syed, Christy Thomas, Bavneet Kaur
Oct 23, 2026