Synthesis Docking Studies, Characterization and Anti-tubercular Activity of Ofloxacin Containing Thiazolidinone Derivatives

Background: Ofloxacin is a fluoroquinolone antibiotic very useful in the treatment of several bacterial infections. The Thiazolidinone ring system represents a privileged structure in drug discovery. multi-drug-resistant tuberculosis bacteria infects many people worldwide and the disease kills 1.8 million people annually, so a combination of ofloxacin-thiazolidinone gives a great promise for future potential antitubercular drugs. Methods: Ofloxacin is treated with hydrazine hydrate results in the form of ofloxacin hydrazides. These hydrazides were treated with various aromatic aldehydes form ofloxacin containing Schiff bases. These compounds are treated with thioglycolic acid in presence of zinc chloride, DMF as solvent forms the ofloxacin containing thiazolidinone derivatives. These derivatives (T1, T2, T3, T4, T5, T6) were purified using Ethanol by recrystallization procedure. Chimera software is used for protein stabilization. The protein 5BS8 was downloaded from PDB. Results: these derivatives are characterized by TLC, melting point, solubility and Spectroscopic methods like FT-IR, 1 H-NMR and HRMS. Predicted in silico studies using Autodock vina, which is academic free version software and performed antitubercular action of synthesized compounds using standard drugs. The concentrations were prepared (0.8, 1.6, 3.12, 6.25, 12.5, 25, 50, 100 µg/ml) and performed antitubercular Assay using the Microplate Alamar Blue Assay method (MABA method). Docking studies were done using autodock vina free software. Conclusion: MIC values reveal that all the synthesized derivatives show the activity in the concentration 1.6 µg/ml compared to standard drugs. the docking studies results that all compounds from T1-T6 showed good binding energies above the standard, the results reveal that these compounds are potent antitubercular agents.