Exploring the Antioxidant Properties and Bioactive Compounds of Methanolic Extract of Diplocyclos palmatus Seeds

Background: Concerns regarding the detrimental consequences of oxidative stress on human health have grown recently on a global scale and herbal treatment approaches to treat oxidative stress-related illnesses in humans are now a hot topic in traditional medicine. Diplocyclos palmatus is one such herbal remedy, it is utilised to treat a wide range of illnesses because of its antioxidant qualities. To provide scientific evidence for this claim the study was planned to evaluate the antioxidant capabilities of D. palmatus Seed Methanolic Extract (SME) using both in vitro and in vivo investigations. Materials and Methods: SME prepared with cold percolation method was first evaluated for phytochemical constituents. In vitro assays were carried out to get a preliminary indication of the antioxidant capacity of extract in a regulated environment whereas, in vivo analysis was done using an induced Wistar rat model to justify its biological relevance. The main bioactive components in SME were identified using the RP-HPLC technique. Results: SME has proven to be an effective antioxidant in both in vitro and in vivo experiments. The RP-HPLC analysis has revealed the presence of quercetin and vanillic acid as the main antioxidants in SME. All the results were obtained as Mean±Standard Deviation. Tests including Student’s T-test (unpaired) and ANOVA were applied to compare the Statistical difference. A ‘p’ value of less than 0.05 was considered significant. Conclusion: The research findings indicate that D. palmatus SME demonstrates noteworthy antioxidant properties. Thus, the findings could contribute to developing a safe and effective natural health supplement.