Open access · Peer reviewed · No APCs
Short communicationJournal of Young PharmacistsVol. 5 | Issue 3 | 2013 | pp. 98–101Open access
Simple and sensitive method for the analysis of ketorolac in human plasma using high-performance liquid chromatography
- 1*,
- 2,
- 3
- 1 Unit of Pharmaceutical Chemistry, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.
- 2 Unit of Pharmaceutical Technology, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.
- 3 Unit of Pharmacology, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.
Published in Journal of Young Pharmacists
Correspondence: Selvadurai Muralidharan
Unit of Pharmaceutical Chemistry, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.
Email: murali23pharm@hotmail.com
Copyright: © 2013 Manuscript Technomedia. This is an open access article.
- Published:
- Aug 18, 2013
- Received:
- May 23, 2013
- Accepted:
- Jul 22, 2013
How to cite
Muralidharan, S., kumar, K. J., & Parasuraman, S. (2013). Simple and sensitive method for the analysis of ketorolac in human plasma using high-performance liquid chromatography. Journal of Young Pharmacists, 5(3), 98–101. https://doi.org/10.1016/j.jyp.2013.06.007
Abstract
Objective: To develop a simple and sensitive method of ketorolac in drug free human plasma using high-performance liquid chromatographic (HPLC). Method: Ketorolac from blank plasma was extracted using protein precipitation method. Trichloro acetic acid (10%) was used as a protein precipitating agent and the percentage of recovery was calculated by adding known volume of dexibuprofen as internal standard. The HPLC separation was achieved on reversed-phase C18 column and the separations were detection by Photodiode Array (PDA) detector at 306 nm. Acetonitrile and 5 mM ammonium acetate (pH 3.5), in the ratio of 60:40% v/v was used as mobile phase at the isocratic flow rate of 1.0 ml/min. The method was validated according to FDA Guidance for Industry. Result: The percentage of recovery of ketorolac was 93.7(+-)0.12 (mean(+-)SD; n ¼ 5). The linearity was achieved in this method range of 10.0e125.0 ng/ml with regression coefficient range of 0.999. Conclusion: Ketorolac bioanalytical method was developed and validated according to FDA Guidance for Industry.
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Article metadata
| Title | Simple and sensitive method for the analysis of ketorolac in human plasma using high-performance liquid chromatography |
|---|---|
| Authors | Selvadurai Muralidharan; Kalaimani Jayaraja kumar; Subramani Parasuraman |
| Affiliations | Unit of Pharmaceutical Chemistry, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.; Unit of Pharmaceutical Technology, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.; Unit of Pharmacology, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA. |
| Corresponding author | murali23pharm@hotmail.com |
| Journal | Journal of Young Pharmacists |
| Volume / Issue | Vol. 5, Issue 3 (2013) |
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