Method development and validation of Bio Flavanoid- Morin Hydrate by RP-HPLC in Human Plasma

Objective: To develop a new, rapid and sensitive RP-HPLC method for the estimation of morin in human plasma by protein precipitation method (PPT) because of more recovery. Method: Chromatographic separation was carried out on a reverse phase C18 column using mixture of 10 mM potassium dihydrogen phosphate (pH 5.0) and acetonitrile (60:40, v/v) at a flow rate of 1.0 ml/min with UV detection at 260 nm. The method was validated and found to be linear in the range of 100 -500 ng/ml. Results: The degree of reproducibility of the results obtained as a result of small deliberate variations in the method parameters and by changing analytical operator has proven that the method is robust and rugged. The present method is suitable in terms of precise, accurate and specific during the study. Conclusion: The simplicity of the method allows for application in laboratories that lack sophisticated analytical instruments which are complicated, costly and time consuming rather than a simple HPLC–UV method. The present method was successfully applied for bioavailability and bioequivalence studies and pharmacokinetic profile studies.