Phytochemical Screening, Acute and Sub‑Acute Toxicity of Aqueous Extract from a Mixture of Some Recipe of Herniaria glabra L., Opuntia ficus‑indica, Zea mays L. and Zizyphus lotus L. Used Traditionally against Renal Lithiases

Introduction: Antiurolithiatic plants are used since ancient times, in the form of decoction, infusion, or juice, to eliminate kidney stones and to prevent their recurrence. On the basis of the available ethnopharmacological information, more scientific studies are needed to explore natural and safe antiurolithiatic compounds. Materials and Methods: The present work showed phytochemical screening, acute and sub‑acute toxicity of aqueous extract of four plant’s mixture: Aerial part (branches, flowers and leaves) of Herniaria glabra, flowers of Opuntia ficus‑indica, Zea mays styles and fruits of Zizyphus lotus used traditionally against renal lithiases. Phytochemical screening was performed using qualitative methods. To measure acute toxicity, rats were administered orally by single doses of 0.1, 0.2, 0.5 and 2.0 g/kg body weight (b. w.) of extract of plant’s mixture. General behavior adverse effects and mortality were determined during 15 days. For sub‑acute study, the aqueous extract was administered at 100 mg/kg b. w. for 28 days to Wistar rats. Animals were monitored daily after an oral administration of aqueous extract of the mixture to detect any changes in b. w., behavior, autonomic profiles, or mortality. Calculation of relative organ weight (ROW) and biochemical analysis were carried out. Results: The acute oral toxicity study showed no mortality and no statistically significant decrease in b. w. and ROW of the treated groups of rats when compared to the control group was observed. In biochemical analysis, there was a significant increase in aspartate aminotransferase, creatinine, urea, and uric acid. Conclusion: This study found that aqueous extract of traditional recipe used against renal lithiasis in Fes‑Meknes region containing: Flavonoids, tannins, catechic tannins, coumarins and the glycosides. The results of the acute and sub‑acute toxicity studies indicated that the recipe extracts induce a slight hepatotoxic effects in rats treated orally with 100 mg/kg (b. w.).