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Original ArticlePharmacognosy ResearchVol. 13 | Issue 1 | 2021 | pp. 28–33Open access
Antiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model
- 1,
- 2*,
- 3,
- 4,
- 5,
- 6,
- 1
- 1 Faculty of Health Sciences, Federal University of Grande Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
- 2 Faculty of Exact Sciences and Technology, Postgraduate Program in Chemistry, Federal University of Grande Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
- 3 Faculty of Pharmaceutical Sciences, Federal University of Mato Grosso do Sul, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
- 4 Faculty of Medicine (FAMED) “Dr. Helio Mandetta”, Federal University of Mato Grosso do Sul,, Campo Grande, MS, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
- 5 Center of Studies in Natural Resources, State University of Mato Grosso do Sul, Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
- 6 Department of Morphology, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
Published in Pharmacognosy Research
Correspondence: Maria Helena Verdan
Faculty of Exact Sciences and Technology, Postgraduate Program in Chemistry, Federal University of Grande Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
Email: mhelenaverdan@gmail.com
Copyright: © 2021 Manuscript Technomedia. This is an open access article.
- Published:
- Apr 1, 2021
- Received:
- Aug 7, 2020
- Accepted:
- Jan 5, 2021
- DOI:
- 10.4103/pr.pr_79_20
How to cite
Branquinho, L. S., Verdan, M. H., Silva-Filho, S. E., Oliveira, R. J., Cardoso, C. A. L., Arena, A. C., & Kassuya, C. A. L. (2021). Antiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model. Pharmacognosy Research, 13(1), 28–33. https://doi.org/10.4103/pr.pr_79_20
Abstract
Objectives: The folk use of Doliocarpus dentatus for pain and inflammatory conditions led our group to evaluate the anti‑inflammatory, antinociceptive, and antiarthritic effects of its ethanol extract from the leaves (EEDd) on mouse models. Results: Oral treatments with EEDd (100–300 mg/kg) significantly inhibited the formalin‑induced nociceptive and cold sensitivity, prevented acetic acid‑induced nociceptive behavior, and prevented articular inflammation (including knee edema, leukocyte infiltration, and mechanical hyperalgesia) induced by zymosan. In the peritonitis model, betulinic acid (BA, 0.3–30 mg/kg) and EEDd (300 mg/kg) significantly inhibited zymosan‑induced leukocyte infiltration. In the complete Freund adjuvant (CFA) model, oral treatments with EEDd (100–300 mg/kg) for 21 days significantly inhibited mechanical hyperalgesia, cold response, and edema. In the MTT viability assay, EEDd (3–90 μg/mL) did not induce leukocytes cytotoxicity. cytotoxicity. Most models employed male and female Swiss mice or, for the CFA test, C57BL/6 mice. Conclusion: This study demonstrates that EEDd exhibited antinociceptive, antihyperalgesic, and antiarthritic potential in mice and BA contribute for the EEDd observed activities.
Keywords
Subject
Article metadata
| Title | Antiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model |
|---|---|
| Authors | Lidiane Schultz Branquinho; Maria Helena Verdan; Saulo Euclides Silva-Filho; Rodrigo Juliano Oliveira; Claudia Andrea Lima Cardoso; Arielle Cristina Arena; Candida Aparecida Leite Kassuya |
| Affiliations | Faculty of Health Sciences, Federal University of Grande Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.; Faculty of Exact Sciences and Technology, Postgraduate Program in Chemistry, Federal University of Grande Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.; Faculty of Pharmaceutical Sciences, Federal University of Mato Grosso do Sul, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.; Faculty of Medicine (FAMED) “Dr. Helio Mandetta”, Federal University of Mato Grosso do Sul,, Campo Grande, MS, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.; Center of Studies in Natural Resources, State University of Mato Grosso do Sul, Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.; Department of Morphology, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL. |
| Corresponding author | mhelenaverdan@gmail.com |
| Journal | Pharmacognosy Research |
| Volume / Issue | Vol. 13, Issue 1 (2021) |
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