Phyto-mediated Synthesis of Silver Nanoparticles with Afrohybanthus travancoricus Leaf Aqueous Extract and Screening of their in vitro Antioxidant, Anti-Inflammatory, and Anti-diabetic Activities

Aim: There are many uses for silver nanoparticles in the field of biomedicine due to their exceptional properties. A major application of silver nanoparticles is the increase of antibiotics that are clinically helpful against microbes and drug-resistant bacteria. However, environmentally friendly methods for their synthesis must be developed. Objectives: Current study, the major objective of this research is concentrated on to suggest an easy and well-organized biosynthesis of silver nanoparticles by confirmed antioxidant, anti-inflammatory and anti-diabetic activities. The ATSNPs (Afrohybanthus travancoricus silver nanoparticles) was biosynthesized with A. travancoricus plant like capping and reducing agent. Materials and Methods: The ATSNPs aqueous extract were mixed by water extract of 1mM AgNO3 solution led to appearance of greenish brown to dark brown colour indicates the synthesis of SNPs. The silver nanoparticles were characterized by UV-visible spectroscopy, FT-IR, SEM, XRD, and EDX analysis. Antioxidant assay was tested through DPPH and ABTS+ assays, anti-inflammatory assay through albumin denaturation and antidiabetic assay by α-amylase and α-glucosidase activities. Results: A colour change of solution from dark brown to light brown, absorption peak of ATSNPs as 290 to 300 nm in UV-vis spectroscopy. ATSNPs showed 6 intense peaks in FTIR analysis and functional groups like phenols, alcohols, 1° amines, alkynes, aliphatic amines, aromatics, and alkyl halides were identified. In XRD analysis showed 5 intense peaks at 20.8°, 30.3°, 40.1°, 40.9°, 60°. The EDX analysis the presence of Ag at 3 kev and SEM analysis showed the size of ATSNPs ranges between 88 μm – 242 μm. Antioxidant activity was observed the dose dependent manner in both assays. Anti-inflammatory activity of ATSNPs proved maximum inhibition through egg albumin denaturation. Antidiabetic activity exhibits maximum inhibition percentage of α-amylase and α- glucosidase at 10 μg/mL. The lowest IC50 value was noted in α amylase (170 μg/mL) and α glucosidase (134 μg/mL). Conclusion: The obtained results recommended that using ATSNPs, it is likely to represented silver nanoparticles with controlled characteristics and important pharmacological activities.