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Original ArticlePharmacognosy ResearchVol. 8 | Issue 1 | 2016 | pp. 71–77Open access
The Effect of Ultrafine Process on the Dissolution, Antibacterial Activity, and Cytotoxicity of Coptidis rhizoma
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- 1 Department of Medical Microbiology, Medical School, Wuhan University of Science and Technology
- 2 Department of Traditional Chinese Medicine Control, Hubei Institute for Food and Drug Control, Wuhan 430064, China
Published in Pharmacognosy Research
Correspondence: Hai-Ying Deng
Department of Medical Microbiology, Medical School, Wuhan University of Science and Technology
Email: denghaiying@wust.edu.cn
Copyright: © 2016 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2016
How to cite
Jiang, Z. Y., Deng, H. Y., Yu, Z. J., Ni, J. Y., & Kang, S. H. (2016). The Effect of Ultrafine Process on the Dissolution, Antibacterial Activity, and Cytotoxicity of Coptidis rhizoma. Pharmacognosy Research, 8(1), 71–77. https://doi.org/10.4103/0974-8490.171097
Abstract
Background
The dosage of herb ultrafine particle (UFP) depended on the increased level of its dissolution, toxicity, and efficacy.
Objective
The dissolution, antibacterial activity, and cytotoxicity of Coptidis rhizoma (CR) UFP were compared with those of traditional decoction (TD).
Materials and Methods
The dissolution of berberine (BBR) of CR TD and UFP was determined by high-performance liquid chromatography. The antibacterial activity of CR extract was assayed by plate-hole diffusion and broth dilution method; the inhibitory effect of rat serums against bacteria growth was evaluated after orally given CR UFP or TD extract. The cytotoxicity of CR extract was evaluated by 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide assay.
Results
The dissolution amount of BBR from CR UFP increased 6–8-folds in comparison to TD at 2 min, the accumulative amount of BBR in both UFP and TD group increased in a time-dependent manner. The minimal inhibitory concentrations and minimal bactericidal concentrations of CR UFP extract decreased to 1/2~1/4 of those of TD extract. The inhibitory effect of rat serums against bacteria growth decreased time-dependently, and no statistical difference was observed between two groups at each time point. The 50% cytotoxic concentrations of UFP extract increased 1.66~1.97 fold than those of TD.
Conclusions
The antibacterial activity and cytotoxicity of CR UFP increased in a dissolution-effect manner in vitro, the increased level of cytotoxicity was lower than that of antibacterial activity, and the inhibitory effect of rat serums containing drugs of UFP group did not improve.
Keywords
Subject
Article metadata
| Title | The Effect of Ultrafine Process on the Dissolution, Antibacterial Activity, and Cytotoxicity of Coptidis rhizoma |
|---|---|
| Authors | Zhen-Yu Jiang; Hai-Ying Deng; Zhi-Jun Yu; Jun-Yan Ni; Si-He Kang |
| Affiliations | Department of Medical Microbiology, Medical School, Wuhan University of Science and Technology; Department of Traditional Chinese Medicine Control, Hubei Institute for Food and Drug Control, Wuhan 430064, China |
| Corresponding author | denghaiying@wust.edu.cn |
| Journal | Pharmacognosy Research |
| Volume / Issue | Vol. 8, Issue 1 (2016) |
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