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Original ArticleFree Radicals and AntioxidantsVol. 11 | Issue 2 | 2021 | pp. 46–51Open access
Comparative Study of Antioxidant Activities of Selected Medicinal Plants of Shujabad Area in Multan, Pakistan
- 1,
- 2,
- 3,
- 4,
- 5*
- 1 Department of Botany, The Women University Multan, Multan, PAKISTAN.
- 2 Department of Environmental Sciences, The Women University Multan, Multan, PAKISTAN.
- 3 Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences-VVAS, Lahore, PAKISTAN.
- 4 Medicinal Plants Research Laboratory, Department of Plant Biotechnology, Atta Ur Rehman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, PAKISTAN.
- 5 Department of Chemistry, School of Natural Sciences, National University of Sciences and Technology (NUST), Islamabad, PAKISTAN.
Published in Free Radicals and Antioxidants
Correspondence: Mudassir Iqbal
Department of Chemistry, School of Natural Sciences, National University of Sciences and Technology (NUST), Islamabad, PAKISTAN.
Email: mudassir.iqbal@sns.nust.edu.pk
Copyright: © 2021 Manuscript Technomedia. This is an open access article.
- Published:
- Dec 31, 2021
- Received:
- Jul 6, 2021
- Accepted:
- Dec 2, 2021
How to cite
Ghaffar, S., Jabeen, S., Mehmood, T., Hayat, M. Q., & Iqbal, M. (2021). Comparative Study of Antioxidant Activities of Selected Medicinal Plants of Shujabad Area in Multan, Pakistan. Free Radicals and Antioxidants, 11(2), 46–51. https://doi.org/10.5530/fra.2021.2.11
Abstract
Background: In humans, especially in elderly patients, free radicals and oxidative stress are one of the main reasons behind a number of diseases/disorders, such as cardiovascular, pulmonary and neuronal in nature. Therefore, it is imperative to investigate substances/compounds that possess potent free radical scavenging activity, especially, from the indigenous flora. Methods: The present study was aimed to screen the free radical scavenging activity of ethanolic and methanolic leaf extracts of three medicinal plants: Syzygium cumini, Psidium guajava and Callicarpa dichotoma. The antioxidant activity was determined using 1,1-Diphenyl-2-picryl hydroxyl (DPPH) and Hydrogen peroxide (H2O2) scavenging assays. Various concentrations (250, 500, 750 and 1000µg/ml) of plant extract were used to carry out the assays and Ascorbic acid was used as the standard. The free radical quenching potential was expressed in inhibition percentage (%) and concentrations were expressed in µg/ml. Optical density of DPPH and H2O2 was measured using spectrophotometer at 517 and 230 nm, respectively. Results: Results from the DPPH and H2O2 assays showed that antioxidant activities were observed to be highest in P. guajava (89 and 81% respectively) followed by S. cumini (84 and 72% respectively) and C. dichotoma (83 and 70% respectively) in the ethanolic extracts. Conclusion: It is concluded that the selected plant materials used for the study have a powerful antioxidant potential and could be used in various therapeutic and medicinal applications.
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Article metadata
| Title | Comparative Study of Antioxidant Activities of Selected Medicinal Plants of Shujabad Area in Multan, Pakistan |
|---|---|
| Authors | Salma Ghaffar; Shazia Jabeen; Tahir Mehmood; Muhammad Qasim Hayat; Mudassir Iqbal |
| Affiliations | Department of Botany, The Women University Multan, Multan, PAKISTAN.; Department of Environmental Sciences, The Women University Multan, Multan, PAKISTAN.; Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences-VVAS, Lahore, PAKISTAN.; Medicinal Plants Research Laboratory, Department of Plant Biotechnology, Atta Ur Rehman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, PAKISTAN.; Department of Chemistry, School of Natural Sciences, National University of Sciences and Technology (NUST), Islamabad, PAKISTAN. |
| Corresponding author | mudassir.iqbal@sns.nust.edu.pk |
| Journal | Free Radicals and Antioxidants |
| Volume / Issue | Vol. 11, Issue 2 (2021) |
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