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Original ArticleFree Radicals and AntioxidantsVol. 7 | Issue 1 | 2016 | pp. 123–127Open access
Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves
- 1*,
- 2,
- 3,
- 1*
- 1 Department of Pharmacology, JSS College of Pharmacy, JSS University, Mysuru, Karnataka, INDIA..
- 2 Department of Pharmacognosy, JSS College of Pharmacy, JSS University, Mysuru, Karnataka, INDIA..
- 3 Department of Biochemistry, Center of Excellence in Molecular Biology and Regenerative Medicine (CEMR), JSS Medical College, Mysuru, Karnataka, INDIA..
Published in Free Radicals and Antioxidants
Correspondence: Anjali Raj
Department of Pharmacology, JSS College of Pharmacy, JSS University, Mysuru, Karnataka, INDIA..
Email: snmanjula@jssuni.edu.in
Copyright: © 2016 Manuscript Technomedia. This is an open access article.
- Published:
- Sep 1, 2016
How to cite
Raj, A., Mruthunjaya, K., Madhunapantula, S. R. V., & Manjula, S. N. (2016). Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves. Free Radicals and Antioxidants, 7(1), 123–127. https://doi.org/10.5530/fra.2017.1.18
Abstract
Objective: The present study was conducted to investigate the antioxidant and anti-clastogenic activity of fractions extracted from Morusalba (Moraceae) leaves. Methods: The leaves of Morus alba were sequentially extracted using n-Hexane, Petroleum Ether, Ethyl Acetate, Methanol and Water, and Total phenolic content was determined using Folin-Ciocalteau (FC) reagent. The anti-oxidant property of each fraction was determined using 2-Deoxy-D-ribose, 2, 2-Diphenyl-1-picryl-hydrazyl (DPPH) and Hydroxyl radical scavenging assay. Furthermore, anti-clastogenic potential of these fractions was determined using calf-thymus DNA in the presence of Fenton’s reagent followed by analyzing the samples by agarose gel electrophoresis. Results: All fractions showed the presence of varied amounts of total phenols and exhibited anti-oxidant activity as determined by scavenging the corresponding radicals. Among these fractions the methanol fraction (MEMA) showed the maximum phenol content as well as anti-oxidant activity. In addition MEMA could inhibit the breakage of DNA strands (anti-clastogenic activity) compared to other fractions tested. Discussion: Results of this study suggests that MEMA is likely to possess better antioxidant and anti-clastogenic activity.
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Article metadata
| Title | Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves |
|---|---|
| Authors | Anjali Raj; Kenganora Mruthunjaya; Subba Rao Venkata Madhunapantula; Santhepete Nanjundaiah Manjula |
| Affiliations | Department of Pharmacology, JSS College of Pharmacy, JSS University, Mysuru, Karnataka, INDIA..; Department of Pharmacognosy, JSS College of Pharmacy, JSS University, Mysuru, Karnataka, INDIA..; Department of Biochemistry, Center of Excellence in Molecular Biology and Regenerative Medicine (CEMR), JSS Medical College, Mysuru, Karnataka, INDIA.. |
| Corresponding author | snmanjula@jssuni.edu.in |
| Journal | Free Radicals and Antioxidants |
| Volume / Issue | Vol. 7, Issue 1 (2016) |
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- In vitro Antioxidant and Cytotoxicity Analysis of Leaves of Ficus racemosapp. 8–12
- In vitro Antioxidant and RBC membrane Stabilization Activity of Euphorbia wallichiipp. 13–22
- Isolation, Structure Elucidation of Ferulic and Coumaric acids from Fortunella japonica Swingle leaves and their Structure Antioxidant activity relationshippp. 23–30
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