Pharmaceutical ResearchIndian Journal of Pharmaceutical Education and ResearchVol. 49 | Issue 4 | 2015 | pp. 282–292Open access
Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy
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- 1 Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712- 749, KOREA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Yong-Ha Park
Email: peter@ynu.ac.kr
Copyright: © 2015 Manuscript Technomedia. This is an open access article.
- Published:
- May 25, 2015
- Received:
- Jan 5, 2015
- Accepted:
- Apr 23, 2015
- DOI:
- 10.5530/ijper.49.4.6
How to cite
Seo, B. J., Bajpai, V. K., Rather, I. A., & Park, Y. H. (2015). Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy. Indian Journal of Pharmaceutical Education and Research, 49(4), 282–292. https://doi.org/10.5530/ijper.49.4.6
Abstract
This study was aimed to partially purify the exopolysaccharide (EPS) from the culture of Lactobacillus plantarum YML009 using ethanol precipitation method with a yield of 260 mg/L. Analytical evaluationby Bradford and Phenolsulphuric methods revealed the presence of 2.2% and 68.1% total protein and total sugar contents in partially purified EPS, respectively. Further, to confirm the biological potential, the EPS was evaluated for its antioxidant activity in various scavenging models including DPPH and nitrite radicals as well as reducing power ability. The partially purified EPS (5-40 mg/mL) displayed considerable amount of antioxidant efficacy on scavenging DPPH and nitrite radicals by 44.73%, and 43.93%, respectively. Moreover, EPS showed potentreducing power capacity along with total phenolic content 18.96 μg/mg of GAE.
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Article metadata
| Title | Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy |
|---|---|
| Authors | Byoung-Joo Seo; Vivek Kumar Bajpai; Irfan Ahmad Rather; Yong-Ha Park |
| Affiliations | Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712- 749, KOREA. |
| Corresponding author | peter@ynu.ac.kr |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 49, Issue 4 (2015) |
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