Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 51 | Issue 4 | 2017 | pp. 597–602Open access
Silibinin: An Inhibitor of Mir-181a Gene Expression in Sk-Br-3 Breast Cancer Cell Line
- 1,
- 2*,
- 2,
- 3
- 1 Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, IRAN.
- 2 School of Biology, College of Science, University of Tehran, Tehran, IRAN.
- 3 Faculty of Medicine, Shahrood Islamic Azad University of Medicine, Shahrood, IRAN.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Nasrin Motamed
School of Biology, College of Science, University of Tehran, Tehran, IRAN.
Email: motamed2@khayam.ut.ac.ir
Copyright: © 2017 Manuscript Technomedia. This is an open access article.
- Published:
- Jul 22, 2017
- Received:
- Jan 6, 2017
- Accepted:
- Jul 13, 2017
How to cite
Shahinfar, P., Motamed, N., Birjandian, E., & Shabanpour, O. (2017). Silibinin: An Inhibitor of Mir-181a Gene Expression in Sk-Br-3 Breast Cancer Cell Line. Indian Journal of Pharmaceutical Education and Research, 51(4), 597–602. https://doi.org/10.5530/ijper.51.4.89
Abstract
Background: Mir-181a has been considered as an attractive molecular target for breast cancer therapy. The main objective of this work is to assess the inhibitory effects of silibinin, a herbal substance, on proliferation, apoptosis and Mir-181a gene expression in human breast cancer cell line SK-BR-3. Materials and Methods: Human breast cancer cell line SK-BR-3 was treated with various concentration of silibinin. Cell viability was assessed by MTT assay and apoptosis by flow cytometry. Expression of human mir-181a gene was measured with real-time PCR. Results: Silibinin inhibits growth of SK-BR-3 cells in a dose- and time-dependent manner and effectively induces apoptosis of SK-BR-3 cells. In addition, silibinin caused a decrease in Mir-181a level. Conclusion: These findings suggest that cell viability along with Mir-181a gene expression in breast cancer cells could be reduced by silibinin. These results suggest that silibinin inhibits the proliferation of SK-BR-3 cells, and it induces apoptosis by down-regulating Mir-181a expression in breast cancer cells.
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Article metadata
| Title | Silibinin: An Inhibitor of Mir-181a Gene Expression in Sk-Br-3 Breast Cancer Cell Line |
|---|---|
| Authors | Parvaneh Shahinfar; Nasrin Motamed; Elnaz Birjandian; Omid Shabanpour |
| Affiliations | Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, IRAN.; School of Biology, College of Science, University of Tehran, Tehran, IRAN.; Faculty of Medicine, Shahrood Islamic Azad University of Medicine, Shahrood, IRAN. |
| Corresponding author | motamed2@khayam.ut.ac.ir |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 51, Issue 4 (2017) |
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