Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 52 | Issue 2 | 2018 | pp. 284–292Open access
Comparison of Mean Centering of Ratio Spectra Based Spectrophotometric Approach and HPLC Method for Quantitative Determination of Pirenoxine in the Presence of Methylparaben and Propylparaben
- 1,2,
- 1,
- 3,
- 1,
- 4,5,
- 1,4,6*
- 1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University; 447 Sri-Ayuthaya Road, Phayathai, Ratchathewi, Bangkok, THAILAND.
- 2 Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University; Dr. Soeparno Road, Karangwangkal, Purwokerto, INDONESIA.
- 3 Department of Health-Related Informatics, Faculty of Pharmacy, Silpakorn University; Nakornpathom Campus, 6 RajamankhaNai Road, Phrapathom Chedi, Muang, Nakorn Pathom, THAILAND.
- 4 Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND.
- 5 National Doping Control Center, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND.
- 6 Center of Excellence for Innovation in Drug Design and Discovery, Faculty of Pharmacy, Mahidol University, Bangkok, THAILAND.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Chutima Matayatsuk Phechkrajang
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University; 447 Sri-Ayuthaya Road, Phayathai, Ratchathewi, Bangkok, THAILAND.; Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND.; Center of Excellence for Innovation in Drug Design and Discovery, Faculty of Pharmacy, Mahidol University, Bangkok, THAILAND.
Email: chutima.mat@ma hidol.ac.th
Copyright: © 2018 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 28, 2018
- Received:
- Jul 20, 2017
- Accepted:
- Oct 3, 2017
How to cite
Wasito, H., Buranaphalin, S., Sratthaphut, L., Suntornsuk, L., Wilairat, P., & Phechkrajang, C. M. (2018). Comparison of Mean Centering of Ratio Spectra Based Spectrophotometric Approach and HPLC Method for Quantitative Determination of Pirenoxine in the Presence of Methylparaben and Propylparaben. Indian Journal of Pharmaceutical Education and Research, 52(2), 284–292. https://doi.org/10.5530/ijper.52.2.33
Abstract
Objective: The mean centering of ratio spectra method (MCR) was developed for determination of pirenoxine in the presence of methylparaben and propylparaben. Background: The UV spectrum of pirenoxine was suffered from spectra overlapping of methylparaben and propylparaben, the preservatives used in the eye drop formulation. Since, MCR method was introduced to overcome this limitation. Methods: The developed MCR method was performed using 39 synthetic mixtures of pirenoxine, methylparaben and propylparaben. The amplitudes at 320 nm of the second ratio spectra were used to construct a calibration model for pirenoxine. Performance characteristics of the method such as linearity, accuracy and precision, were calculated. A high-performance liquid chromatography (HPLC) method was also developed and validated. Then, two methods were used to determine a set of commercial eye drop samples for comparison. Results: The developed and validated MCR method was simple, rapid, accurate, and precise and could be applied to determine pirenoxine in eye drop samples. Measurement of pirenoxine in eye drop samples by MCR and HPLC methods were not significantly different (P-value = 0.21).
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Article metadata
| Title | Comparison of Mean Centering of Ratio Spectra Based Spectrophotometric Approach and HPLC Method for Quantitative Determination of Pirenoxine in the Presence of Methylparaben and Propylparaben |
|---|---|
| Authors | Hendri Wasito; Sawanya Buranaphalin; Lawan Sratthaphut; Leena Suntornsuk; Prapin Wilairat; Chutima Matayatsuk Phechkrajang |
| Affiliations | Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University; 447 Sri-Ayuthaya Road, Phayathai, Ratchathewi, Bangkok, THAILAND.; Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University; Dr. Soeparno Road, Karangwangkal, Purwokerto, INDONESIA.; Department of Health-Related Informatics, Faculty of Pharmacy, Silpakorn University; Nakornpathom Campus, 6 RajamankhaNai Road, Phrapathom Chedi, Muang, Nakorn Pathom, THAILAND.; Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND.; National Doping Control Center, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND.; Center of Excellence for Innovation in Drug Design and Discovery, Faculty of Pharmacy, Mahidol University, Bangkok, THAILAND. |
| Corresponding author | chutima.mat@ma hidol.ac.th |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 52, Issue 2 (2018) |
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