Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 52 | Issue 4 | 2018 | pp. s133–s139Open access
Detection of the Genotoxicity of Gentiana L. Extracts by Using RAPD-PCR and ISSR-PCR Techniques
- 1*,
- 2,
- 3,
- 3,
- 4,
- 4,
- 5
- 1 Department of Pharmaceutical Botany, Anadolu University, 26470 Eskişehir, TURKEY.
- 2 Department of Molecular Biology and Genetics, Bilecik Şeyh Edebali University, 11210 Bilecik, TURKEY.
- 3 Department of Pharmacognosy, Anadolu University, 26470 Eskişehir, TURKEY.
- 4 Republic of Turkey Ministry of Food, Agriculture and Livestock, Atatürk Horticultural Central Research Institute, Yalova, TURKEY.
- 5 Department of Horticulture, Ege University, 35100 İzmir, TURKEY.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: İlham Eröz Poyraz
Department of Pharmaceutical Botany, Anadolu University, 26470 Eskişehir, TURKEY.
Email: ieroz@anadolu.edu.tr
Copyright: © 2018 Manuscript Technomedia. This is an open access article.
- Published:
- Jun 20, 2018
- Received:
- Aug 30, 2016
- Accepted:
- Nov 23, 2016
How to cite
Poyraz, İ. E., Poyraz, İ., Kıyan, H. T., Öztürk, N., Erken, S., Gülbağ, F., & Özzambak, M. E. (2018). Detection of the Genotoxicity of Gentiana L. Extracts by Using RAPD-PCR and ISSR-PCR Techniques. Indian Journal of Pharmaceutical Education and Research, 52(4), s133–s139. https://doi.org/10.5530/ijper.52.4s.89
Abstract
Background: The RAPD- and ISSR-PCR techniques are offering an insight into the detecting the genotoxicity of Gentiana extracts. Objective: It is aimed with the present study, to detect the genotoxicity of methanol extracts of ten Turkish Gentiana L. taxa on germinated Allium cepa L. root tips. Methods: RAPD- and ISSR-PCR techniques were used for detection of genotoxicity of Gentiana extracts. Results: Four RAPD and three ISSR primers produced the reproducible polymorphic and monomorphic banding patterns among 10 RAPD and 10 ISSR primers for all DNA samples. It is not any serious alteration along with band intensity change, the disappearance of the bands, and appearance of the new bands in the band profiles amplified from the Gentiana extracts-treated genomic DNA sample of A. cepa. The most efficient results were obtained by RAPD-P9 and ISSR-1 primer among the seven productive primers. Discussion: It is not observed any variation in the RAPD- and ISSR-PCR band profiles in time and concentration-dependent manner. Conclusion: It is determined that the three different concentrations of Gentiana extracts did not interact with the A. cepa DNA.
Keywords
Subject
Article metadata
| Title | Detection of the Genotoxicity of Gentiana L. Extracts by Using RAPD-PCR and ISSR-PCR Techniques |
|---|---|
| Authors | İlham Eröz Poyraz; İsmail Poyraz; H. Tuba Kıyan; Nilgün Öztürk; Serdar Erken; Fatih Gülbağ; M. Ercan Özzambak |
| Affiliations | Department of Pharmaceutical Botany, Anadolu University, 26470 Eskişehir, TURKEY.; Department of Molecular Biology and Genetics, Bilecik Şeyh Edebali University, 11210 Bilecik, TURKEY.; Department of Pharmacognosy, Anadolu University, 26470 Eskişehir, TURKEY.; Republic of Turkey Ministry of Food, Agriculture and Livestock, Atatürk Horticultural Central Research Institute, Yalova, TURKEY.; Department of Horticulture, Ege University, 35100 İzmir, TURKEY. |
| Corresponding author | ieroz@anadolu.edu.tr |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 52, Issue 4 (2018) |
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