Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 53 | Issue 2s | 2019 | pp. s179–s185Open access
In vitro Metabolism of Risperidone on Isolated Microsomes of Rat Liver: Metabolite Identification and Profiling by RP-HPLC and LC-MS Techniques
- 1*,
- 2,
- 3,
- 1
- 1 Department of Pharmaceutical Chemistry, S.M.B.T. College of Pharmacy, Nashik, Maharashtra, INDIA.
- 2 Department of Pharmaceutical Chemistry, Sandip Foundation’s, Sandip Institute of Pharmaceutical Sciences, Nashik, Maharashtra.
- 3 Department of Pharmaceutics, Sandip Foundation’s, Sandip Institute of Pharmaceutical Sciences, Nashik, Maharashtra, INDIA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Prutha Shirodkar
Department of Pharmaceutical Chemistry, S.M.B.T. College of Pharmacy, Nashik, Maharashtra, INDIA.
Email: shirodkarprutha@gmail.com
Copyright: © 2019 Manuscript Technomedia. This is an open access article.
- Published:
- Apr 12, 2019
- Received:
- Jul 7, 2018
- Accepted:
- Jan 22, 2019
How to cite
Shirodkar, P., Chavan, H., Mogal, R., & Dhake, A. (2019). In vitro Metabolism of Risperidone on Isolated Microsomes of Rat Liver: Metabolite Identification and Profiling by RP-HPLC and LC-MS Techniques. Indian Journal of Pharmaceutical Education and Research, 53(2s), s179–s185. https://doi.org/10.5530/ijper.53.2s.64
Abstract
Objective: The objective of present research work is to study in vitro metabolism of risperidone on isolated microsomes of rat liver. Methods: Its biotransformation was examined using isolated microsomes derived from rat livers. Their separation was accomplished on Jasco RP-HPLC C-18 column (250mm x 4.6mm x 5µm) using methanol: 50mM Potassium dihydrogen phosphate (50:50 %v/v) mobile phase, detected at a wavelength of 280nm. Results: In vitro metabolism of RIS was investigated using the cytochrome P450 (CYP450) enzymes supplemented with an NADPH- generating system. Incubation of RIS with rat liver microsomes resulted in the formation of 2 major hydroxy metabolites. Conclusion: Formation of all the metabolites is correlated with Phase I biotransformation CYP enzyme subfamilies. The method was extended to LC-MS for further characterization of the metabolites using standards and the probable metabolites were proposed.
Keywords
Subject
Article metadata
| Title | In vitro Metabolism of Risperidone on Isolated Microsomes of Rat Liver: Metabolite Identification and Profiling by RP-HPLC and LC-MS Techniques |
|---|---|
| Authors | Prutha Shirodkar; Harshal Chavan; Rajendra Mogal; Avinash Dhake |
| Affiliations | Department of Pharmaceutical Chemistry, S.M.B.T. College of Pharmacy, Nashik, Maharashtra, INDIA.; Department of Pharmaceutical Chemistry, Sandip Foundation’s, Sandip Institute of Pharmaceutical Sciences, Nashik, Maharashtra.; Department of Pharmaceutics, Sandip Foundation’s, Sandip Institute of Pharmaceutical Sciences, Nashik, Maharashtra, INDIA. |
| Corresponding author | shirodkarprutha@gmail.com |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 53, Issue 2s (2019) |
Also in this issue
- Recent Developments on Pharmacological Potential of 1,3,4-Oxadiazole Scaffoldpp. s1–s16
- Mucoadhesive Nanoparticles: A Roadmap to Encounter the Challenge of Rapid Nasal Mucociliary Clearancepp. s17–s27
- Sesamol and Health – A Comprehensive Reviewpp. s28–s42
- Novel Preparation and Effective Delivery of Mucoadeshive Nanoparticles Containing Anti-diabetic Drugpp. s43–s49
- Formulation and Characterization of Controlled Release Flurbiprofen Microsponges Loaded in Gelspp. s50–s57
Readers Also Viewed
Effect of the Fractions of Coccinia grandis on Ethanol- Induced Cerebral Oxidative Stress in Rats
M. Umamaheswari, T. K. Chatterjee
Jan 1, 2009
Phytochemical Screening and Antioxidant Activity Evaluation of Selected Philippine Fruit Peels and Pulps
Mildred Dagcuta Pates, Angelo Mark Po Walag, Romeo M. Del Rosario
Aug 17, 2026
Impact of Artificial Intelligence Assisted Drug Therapy Optimization on Drug Related Problems Amongst Older Adult Patients: A Systematic Review
Jehath Syed, Christy Thomas, Bavneet Kaur
Oct 23, 2026