Open access · Peer reviewed · No APCs
Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 54 | Issue 2 | 2020 | pp. 465–470Open access
HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2
- 1*,
- 2,
- 2,
- 1
- 1 Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
- 2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
Published in Indian Journal of Pharmaceutical Education and Research
Correspondence: Vania Maslarska
Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
Email: vmaslarska@mail.bg
Copyright: © 2020 Manuscript Technomedia. This is an open access article.
- Published:
- Mar 12, 2020
- Received:
- Aug 23, 2019
- Accepted:
- Jan 8, 2019
How to cite
Maslarska, V., Amerikova, M., Pencheva, I., & Bozhanov, S. (2020). HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2. Indian Journal of Pharmaceutical Education and Research, 54(2), 465–470. https://doi.org/10.5530/ijper.54.2.53
Abstract
Aim: The aim of this research study was to develop a simple, rapid, precise, accurate and economical RP-HPLC method, with a simple mobile phase for the identification and determination of the antimicrobial peptide α-defensin-2. Materials and Methods: Separation was carried out at 25°C, using column Luna 5U (C18, 250x4.6, 5 μm) with mobile phase consisting of acetonitrile: water (40: 60 v/v). The detector was set at 210 nm. The flow rate was 1.2 ml/min and injection volume was 20 μl. Results: The developed method was validated as per ICH guidelines and the kinetic behavior of α-defensin 2 was studied in respect of different pH. Conclusion: The obtained data can serve for creating an in vivo monitoring program and quality control for different studies and preparations.
Keywords
Subject
Article metadata
| Title | HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2 |
|---|---|
| Authors | Vania Maslarska; Meri Amerikova; Ivanka Pencheva; Stanislav Bozhanov |
| Affiliations | Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA. |
| Corresponding author | vmaslarska@mail.bg |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume / Issue | Vol. 54, Issue 2 (2020) |
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