A New RP-HPLC Method for Simultaneous Estimation of Nebivolol Hydrochloride and Hydrochlorthiazide in Dosage Forms

Y, Gupta; A, Shrivastava; D, Duggal; A, Patel; S, Agrawal

doi:10.4103/0975-1483.57078

Pharmaceutical AnalysisJournal of Young PharmacistsVol. 1 | Issue 3 | 2009 | pp. 264–269Open access

A New RP-HPLC Method for Simultaneous Estimation of Nebivolol Hydrochloride and Hydrochlorthiazide in Dosage Forms

¹^*,
²,
³,
⁴,
⁵

¹ ADL Department, Wokhardt Ltd, Aurangabad, Maharashtra, INDIA.
² Department of Pharmaceutical Analysis, INDIA.
³ Department of Pharmaceutics, B. R. Nahata College of Pharmacy, Mandsaur, INDIA.
⁴ Zydus Research Center, Ahmedabad, Gujrat, INDIA.
⁵ Department of Q.A, Lachoo College of Science and Technology, Shastri Nagar, Jodhpur, Rajasthan, INDIA.

Correspondence: Gupta Y
ADL Department, Wokhardt Ltd, Aurangabad, Maharashtra, INDIA.
Email: yogeshguptajaipur@gmail.com

Published:: Aug 16, 2009
DOI:: 10.4103/0975-1483.57078

How to cite

Y, G., A, S., D, D., A, P., & S, A. (2009). A New RP-HPLC Method for Simultaneous Estimation of Nebivolol Hydrochloride and Hydrochlorthiazide in Dosage Forms. Journal of Young Pharmacists, 1(3), 264–269. https://doi.org/10.4103/0975-1483.57078

Abstract

A new RP-HPLC method has been developed for the simultaneous estimation of nebivolol hydrochloride and hydrochlorothiazide in pharmaceutical dosage forms, using ultra violet (UV) detector. Elution was carried out using a mobile phase consisting of acetonitrile and potassium dihydrogen phosphate buffer (pH 3.2 ± 0.1) in the ratio of 50:50 v/v and ß ow rate was set on 1.2 ml/min at 282 nm. The retention time for hydrochlorothiazide and nebivolol hydrochloride was found 3.57 and 6.66 mins, respectively. The method was found to be linear in the range of 832 μg/ml and 2080 μg/ml for nebivolol hydrochloride and hydrochlorothiazide, respectively. In the linearity study, regression equation and coefÞ cient of correlation for nebivolol hydrochloride and hydrochlorothiazide were found to be: y = 284761x + 215452, r = 0.9995; and y = 698395+105272, r = 0.9998, respectively.

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Article metadata

Title	A New RP-HPLC Method for Simultaneous Estimation of Nebivolol Hydrochloride and Hydrochlorthiazide in Dosage Forms
Authors	Gupta Y; Shrivastava A; Duggal D; Patel A; Agrawal S
Affiliations	ADL Department, Wokhardt Ltd, Aurangabad, Maharashtra, INDIA.; Department of Pharmaceutical Analysis, INDIA.; Department of Pharmaceutics, B. R. Nahata College of Pharmacy, Mandsaur, INDIA.; Zydus Research Center, Ahmedabad, Gujrat, INDIA.; Department of Q.A, Lachoo College of Science and Technology, Shastri Nagar, Jodhpur, Rajasthan, INDIA.
Corresponding author	yogeshguptajaipur@gmail.com
Journal	Journal of Young Pharmacists
Volume / Issue	Vol. 1, Issue 3 (2009)