Simultaneous Analysis of Lamivudine and Dolutegravir Sodium in Formulation Using First Order Derivative Method

Objective: This present study is focused on the development and validation of first order derivative spectrophotometric method for simultaneous quantification of Lamivudine and Dolutegravir sodium in dosage form. Materials and Methods: The solutions were prepared using distilled water as solvent and were scanned in UV region for first order derivative spectrum. The quantitation of drugs were done at 255.6 nm (ZCP (zero crossing point) of Dolutegravir Sodium) and 268.6 nm (ZCP of Lamivudine) over the concentration range 5–30 μg/ml and 1 – 30 μg/ml for Lamivudine and Dolutegravir Sodium respectively in tablet dosage form. Results: The Lamivudine and Dolutegravir Sodium showed linear response with a correlation coefficient of 0.9998 and 0.9999 correspondingly. The relative standard deviation for precision study was found less than 2 %. The accuracy study was determined to be between 98 and 102 % utilising standard addition method. The limit of detection for Lamivudine and Dolutegravir sodium was found 0.89 µg/ml and 0.28 µg/ml correspondingly. The limit of quantitation for Lamivudine and Dolutegravir sodium was found 2.70 µg/ml and 0.85 µg/ml correspondingly. Conclusion: The proposed approach, validated as per ICH Q2 (R1), was deemed to be accurate, repeatable and precise. The results of proposed method suggests that it can be effectively utilised for simultaneous quantitation of said drugs in formulation.