Simultaneous Estimation of Eugenol and Scopoletin by UV-Spectroscopic Method Using in-house Avipattikar churna

Objectives: In this study, a new brisk, efficient and precise method of first derivative UV spectrophotometric was developed and validated for the estimation of eugenol and scopoletin simultaneously from the in-house Avipattikar churna. Materials and Methods: The method involved estimation of eugenol and scopoletin by simultaneous equation of first derivative spectra at 296 nm and 278.66 nm respectively in 0.1 N HCl. As per the Beer-Lambert law, proportional absorbance (linearity) was recorded in the concentration limit of 20-100μg/mL for both eugenol and scopoletin. Results: The method validation was done in reference to ICH (International council for harmonisation of technical requirements for pharmaceuticals for human use) norms comprising of accuracy, linearity, precision, the limit of detection, and quantification of standard from churna. This method was found to be precise as % relative standard deviation was less than 2%. The mean recoveries of triplicate samples were found in the range of 98.52 – 101.30% and 100.51 – 100.56 % for eugenol and scopoletin respectively. The limit of detection and limit of quantification were found to be 2.62μg/mL and 7.96μg/mL for eugenol respectively. The limit of detection and limit of quantification were found to be 1.54μg/mL 4.66μg/mL for scopoletin respectively. Conclusion: The developed estimation method was found to be specific, rapid, precise, and accurate for the analysis of eugenol and scopoletin in the Ayurvedic churna and in formulation without showing any interference.