Cardioprotective Potential of Cardiboost, a Standardized Extract from Terminalia arjuna Bark against H2O2 Induced Oxidative Stress in H9c2 Cardiomyocytes

Background: Terminalia arjuna is an evergreen tree commonly referred as Arjuna, widely distributed in India and is a prominent member of the genus Terminalia. It has been discovered that the various plant parts, including the root, leaf, bark, fruits, and seeds have unique medicinal property. Among them, bark part is found to have and plethora of bioactive compounds and rich in medicinal property, especially for its cardiovascular properties. Materials and Methods: In the current investigation we have developed a standardized extract of T. arjuna bark called Cardiboost, which contains 1% arjunolic acid. Cardiboost was used to examine in vitro thrombin and ACE enzyme inhibition. Additionally, MTT assay was used to measure cell viability of H9c2 cardiomyocytes, western blot experiments were performed to measure the levels of antioxidative enzyme levels CAT and SOD and the expressions of cell apoptosis regulatory proteins PARP1, Casp antiapoptotic properties to elucidate Cardiboost ability to prevent H9c2 cardiomyocytes cells suffering from oxidative damage induced by H2O2. Results: Cardiboost, exhibited potent thrombin and ACE inhibition property, with IC50 values of 111.88μg/mL and 196.66 μg/mL respectively, Furthermore, Cardiboost, averted the cell death and significantly increased the levels of antioxidant enzymes CAT and SOD which was subsided in presence of H2O2, and Cardiboost protected the mitochondrial events and reduced the apoptotic effects in H2O2-induced oxidative stress. Conclusion: Cardiboost could shield H9c2 cardiomyocytes from oxidative stress and may also be used as a medicinal product to prevent oxidative stress in cardiac damage.