Study on the Safety and Efficacy of Indonesian Combination Plant Extract in Cosmetics

Objective: To look for antioxidant, lightening and anti aging effects from combination of Indonesian plant consist of Centella asiatica, Camellia si nensis and Glycine max extract in propanediol (CCGE). Methodology: The study was carried out using DPPH scavenging activity for antioxidant effect and anti tyrosinase activity for lightening effect. The efficacy test on volun teers was performed using Mexameter MX 16 and Cutometer MPA 580 to measure skin melanin index, elasticity and firmness respectively. The dermatological safety test was conducted using Repeated Opened Patch Test (ROPT) and Single Closed Patch Test (SCPT), while HETCAM test was performed to evaluate irritation at ocular level. Result: At the concentration of 2%, CCGE has DPPH scavenging activity and anti tyrosinase activity of 70.56% and 52.76% respectively compared to ascorbic acid and kojic acid. The efficacy test showed that CCGE significantly increased skin elasticity and firmness after two and four weeks of application compared to base, but showed no significant effect on skin lightening. The dermato logical safety test showed that CCGE did not cause any irritation or allergic skin reaction at the concentration of 5% for the ROPT and up to 3% for the SCPT. Meanwhile at the concentration of 5%, CCGE could cause allergic reaction in 4.0% of all subjects. The HETCAM test showed that CCGE is not irritant at the ocular level. Conclusion: These results concluded that CCGE is proven to be safe for human skin as well as at ocular level and could be used as cosmetic active ingredient for anti aging.