Antioxidant and Anti-Inflammatory Impact of Jujuboside B on LPS-Stimulated RAW 264.7 Cells

Aim: Zizyphus jujuba Mill, a well-known traditional Eastern medicine, has been documented to display a variety of biological system-related actions. Zizyphus jujuba fruits contain a natural saponin called jujuboside B (JB), which is one of their active ingredients. Materials and Methods: In the current work, the viability of the RAW-264.7 cells was assessed by treating them with varying concentrations of JB. Also, measurement of nitric oxide and intracellular ROS generation. We examined the antioxidant enzymes and anti-inflammatory activities of jujuboside B on RAW-264.7 cells stimulated with Lipopolysaccharide (LPS). Results: At every treatment concentration, the cells remained viable. Antioxidant enzyme levels were downregulated after LPS treatment, although NO, ROS, and pro-inflammatory cytokines levels were significantly elevated. This suggests that cells exposed to LPS display signs of inflammation and oxidative damage. Following JB therapy, antioxidant enzyme levels increased due to a considerable reduction in NO, ROS, and pro-inflammatory cytokine levels. This demonstrates JB's anti-inflammatory and antioxidant properties. Conclusion: Our findings provide scientific justification for the utilization of JB for treating inflammatory illnesses by indicating that it modulates the major inflammatory processes and reduces the inflammatory responses stimulated by LPS in RAW 264.7 cells.