In vitro Screening for Antioxidant and Antimicrobial Properties of 3,5-Bis(E-thienylmethylene) piperidin-4-one, a Curcumin Analogue

Background: Curcumin is a naturally occurring bis-chalcone derivative in the commonly used Indian spice turmeric and is well-known for its potent chemopreventive, anti-angiogenic, anti-cancer properties. 3,5-bis[(E)-thienylmethylene] piperidin-4-one (BTMP) is the newly synthesised synthetic analogue of curcumin that is tested for its antioxidant and antimicrobial properties in vitro. Objectives: This study was to determine the antioxidant activity and antibacterial potentials of newly synthesised analogues of curcumin viz., 3,5-bis[(E)- thienylmethylene] piperidin-4-one (BTMP), was examined in vitro. Materials and Methods: BTMP was evaluated for its antioxidant and antimicrobial using 1,1-diphenyl-2-picryl hydroxyl (DPPH•) radical scavenging assay, 2,2’-azinobis3-ethyl benzothiazoline-6-sulfonic acid (ABTS•+), ferric reducing antioxidant power (FRAP), nitric oxide scavenging assay, superoxide anion radical scavenging assay (O2•−), hydrogen peroxide scavenging, reducing ability assay, metal ion chelating and phosphomolybdenum assays. Results: 40μg/ml of BTMP showed higher antioxidant activity than the other three doses due to its highest free radical scavenging ability at this particular dose. All doses of BTMP showed antimicrobial activity against E. coli and S. aureus. 40μg/ml of BTMP showed the highest zone of inhibition against both tested pathogens. Conclusion: The introduction of basic functionality and the presence of exocyclic double bonds of BTMP has significantly contributed to the antioxidant activity and antimicrobial activity against both gram-positive and gram-negative bacteria.