LC-MS and HPTLC Profile of Crude Ethanol Extract of Plant Michelia champaca Linn.

Aim: The plant Michelia champaca L. plant is a very rich source of phytoconstituents with medicinal properties. The aim of the study is to analyze the chemical constituents present in the crude extract responsible for various pharmacological actions. Materials and Methods: The aerial parts of the plant Extracted with ethanol and subjected to LC-MS and HPTLC analysis by using Water Q-TOF micro coupled with 2795 HPLC and CAMAG TLC SCANNER -3. Results: LC-MS analysis showed the various peaks with low and high molecular weights indicating the presence of various phytochemicals. The compounds identified in the ethanol extract include parthenolides, quercetin, stigmasterol, sitosterol, and liriodenine and some unknown compounds by comparison with the SDBS database. HPTLC of the ethanol extract showed the Rf value of five compounds, were found to be more prominent. 0.15, 0.3, 0.45, 0.6, 0.75. identified by comparison with HPTLC - derived database. Conclusion: The compounds identified by LC-MS and HPTLC of the ethanol extract of the aerial plant Michelia champaca Linn. may be responsible for its various pharmacological activities, and hence this plant can be used in the treatment of various ailments.