In vitro Cytotoxicity Evaluation of Hardwickia binata Roxbs against Human Breast Cancer Cell Line MCF-7 and Human Cervical Cancer Cell Line HeLa

Hardwickia binata belongs to the family Caesalpiniaceae is used traditionally folklore remedies for a variety of illnesses, such as fungus, gram-positive and gram-negative bacteria, cancer, diarrhoea, leprosy, worm infections, dyspepsia, leucorrhoea, chronic cystitis and gonorrhoea. In the present study the leaves of Hardwickia binata were treated to solvent extraction using ethyl acetate, chloroform, acetone, ethanol by soxhlet. The various solvent extract were investigated for the presence of phytochemicals such as phenolic substances, flavonoids, tannins, glycosides and proteins. The phytochemical were determined by conventional qualitative test and quantitative test like total phenolic, total flavonoid content. Antioxidant activity was done by different assay method using DPPH radical scavenging assay, nitric acid scavenging, reducing power assay method. Cytotoxicity efficacy was estimated using MTT assay method against human breast cancer cell line MCF-7 and human cervical cancer cell line HeLa. IC₅₀ was calculated. This study showed that Hardwickia binata leaves may have potential to inhibit cancer cell growth in addition to focus on search of phytochemicals from leaves of Hardwickia binata roxbs due to their health promising effect including antioxidant and anticancer and can be used as leading drugs to treat cancer. The plant also has excellent antioxidant activity.