QbD-Driven Development of PACE-GC: A Synergistic Polyherbal Anti-Ageing Cream with Extracts of Grape and Carrot

Background

Skin ageing is a multifactorial biological process influenced by extrinsic factors like UV radiation and intrinsic factors like genetics. Oxidative stress mediated by ROS plays a pivotal role in premature ageing, manifesting as wrinkles and loss of elasticity.

Objectives

This study aims to develop and characterise a polyherbal anti-ageing cream (PACE-GC) incorporating Daucus carota and Vitis vinifera extracts, focusing on antioxidant properties and safety.

Materials and Methods

Aqueous Vitis vinifera and acetone Daucus carota extracts were incorporated into a vanishing cream base using Quality by Design (QbD) methodology. Formulations were evaluated for pH, viscosity, and spreadability. Antioxidant activity was assessed using DPPH and hydrogen peroxide scavenging assays.

Results and Discussion

Vitis vinifera and Daucus carota extracts showed notable antioxidant activity (IC₅₀ of 14.10±0.68 µg/mL and 11.90±0.55 µg/mL, respectively). The polyherbal cream (PACE-GC) displayed acceptable physicochemical properties and skin-compatible pH.

Conclusion

The PACE-GC formulation effectively increases antioxidant capacity and demonstrates a favourable profile for mitigating oxidative stress-induced skin ageing.