Effects of Torilis nodosa [L.] Gaertn. Methanol Extract against Rotenone Induced Parkinson Diseases in Rats

Background

Torilis nodosa [L.] Gaertn. a species of flowering plant in the family Apiaceae has conventionally been used for treatment of many human diseases. Currently we have designed to explore the pharmacological evidence of its in neurodegeneration. In the present study we have aimed to investigate the phytochemical screening, in vitro antioxidant activities and in vivo anti-Parkinson effects of Torilis nodosa methanol fraction against rotenone induced Parkinsonism in rats.

Materials and Methods

Fully matured plant of Torilis nodosa was collected shed dried and extracted with methanol. The crude methanol fraction was further fractionated with various solvents (N-hexane, ethyl acetate, chloroform and butanol) with increasing polarity. Various free radicals were used to investigate antioxidant potential of various fractions. The most active methanol fraction was forwarded for in vivo analysis in rats against rotenone induced Parkinson disease in rats.

Results

Findings of the current study revealed that various fractions of Torilis nodosa composed of flavonoids, alkaloids, terpenoid, saponins, tannins, anthraquinones. Various fractious of Torilis nodosa showed potent in vitro antioxidant activities however methanol fraction revealed high efficacy as compare to other fractions which may be due the presence of high amount of flavonoid and phenolic constituents. Correlation study presented that methanol fraction is markedly correlated with antioxidant activities. The in vivo ani-parkison effects of 150 mg/kg body weight and 300 mg/kg body weight methanol Torilis nodosa showed significant protective effects at behavioral, physiological, and biochemical levels. Brain tissue homogenate showed that Torilis nodosa amended the rotenone-induced aberrations, augmented antioxidant enzyme activity, and abridged lipid peroxidation.

Conclusion

From the findings of the current research, it is inferred that Torilis nodosa (300 mg/kg) revealed more marked results than Torilis nodosa (150 mg/kg). These findings showed that Torilis nodosa may be utilized as precursor in the preparation of a promising drug in reducing the risk and progression of Parkinson's disease after further analysis.