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Original ArticlePharmacognosy ResearchVol. 8 | Issue 1 | 2016 | pp. 37–42Open access
Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography
- 1,2*,
- 3
- 1 Department of Pharmacology, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra
- 2 Ph.D. Research Scholar, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
- 3 Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, Maharashtra, India
Published in Pharmacognosy Research
Correspondence: Gaurav Mahesh Doshi
Department of Pharmacology, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra; Ph.D. Research Scholar, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
Email: gaurav.pharmacology@gmail.com
Copyright: © 2016 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2016
How to cite
Doshi, G. M., & Une, H. D. (2016). Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography. Pharmacognosy Research, 8(1), 37–42. https://doi.org/10.4103/0974-8490.171098
Abstract
Objective
In Indian Ayurvedic system, Benincasa hispida (BH) and Carissa congesta (CC) are well-known plants used for major and minor ailments. BH has been regarded as Kushmanda, whereas CC has been used in immune-related disorders of the human system. Quercetin and rutin identified from the vast plethora of plant extracts have proved to possess ethnopharmacological relevance.
Materials and Methods
In present studies, we have determined quercetin and rutin in terms of percentage in BH seeds and CC roots by high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC). After extraction and phytochemical screening, the extracts were subjected to quantification for the presence of quercetin and rutin by HPTLC and HPLC.
Results
HPTLC showed quercetin as 44.60, 27.13% and rutin as 32.00, 36.31% w/w, whereas HPLC revealed quercetin as 34.00, 35.00% and rutin as 21.99, 45.03% w/v in BH and CC extracts, respectively.
Conclusion
The BH and CC extracts have elucidated peaks that were corresponding with standard peaks on undertaking chromatographic studies.
Keywords
Subject
Article metadata
| Title | Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography |
|---|---|
| Authors | Gaurav Mahesh Doshi; Hemant Devidas Une |
| Affiliations | Department of Pharmacology, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra; Ph.D. Research Scholar, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan; Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, Maharashtra, India |
| Corresponding author | gaurav.pharmacology@gmail.com |
| Journal | Pharmacognosy Research |
| Volume / Issue | Vol. 8, Issue 1 (2016) |
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