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Original ArticlePharmacognosy ResearchVol. 8 | Issue 4 | 2016 | pp. 287–291Open access
Bioactivity of Diterpens from the Ethyl Acetate Extract of Kingiodendron pinnatum Rox. Hams
- 1*,
- 2,
- 3,
- 4
- 1 Department of Clinical and Experimental Medicine, Experimental Haematology Unit, University of Linkoping, Linkoping, Sweden
- 2 Department of Environmental Science
- 3 Department of Biochemistry, PBM Mahajana PG Centre, Mysore
- 4 Department of Biochemistry, University of Mysore, Karnataka, India
Published in Pharmacognosy Research
Correspondence: Komal Kumar Javarappa
Department of Clinical and Experimental Medicine, Experimental Haematology Unit, University of Linkoping, Linkoping, Sweden
Email: kompaddy@yahoo.com
Copyright: © 2016 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2016
How to cite
Javarappa, K. K., Prasad, A. G. D., Prasad, A. M., & Mane, C. (2016). Bioactivity of Diterpens from the Ethyl Acetate Extract of Kingiodendron pinnatum Rox. Hams. Pharmacognosy Research, 8(4), 287–291. https://doi.org/10.4103/0974-8490.188871
Abstract
Background
Kingiodendron pinnatum Rox. Hams. is an endangered medicinal plant used in gonorrhoe, catarrhal conditions of genito-urinary and respiratory tracts. The scientific and pharmacological formulation of K. pinnatum has not been established so far though it is being traditionally used by tribes of the region.
Objective
P hytochemical screening and identification of the bioactive compounds from the ethyl acetate extract of Kingiodendron pinnatum Rox. Hams.
Materials and Methods
Chromatographic separation was carried out by thin layer chromatography and column chromatography. Bio-autography of the column fractioned extract and TLC chromatogram were evaluated in vitro for antibacterial activity. The PTLC, HP TLC were used for crude extract and HPLC, LCMS, FTIR 1HNMR and 13CNMR were employed for the isolated compound in the ethyl acetate extract of K. pinnatum.
Results
Evaluation of solvent system for chromatographic separation revealed that ethyl acetate: petroleum ether in the ratio of 7:2.5 ml was the most appropriate one for the separation of diterpene compounds. The antibacterial bio-autography screening of TLC separated compound showed positive activity with Staphylococcus aureus and negative activity with Escherichia coli. Spectroscopic analysis of the isolated compound from the ethyl acetate extract of K. pinnatum revealed the presence of diterpene compound.
Conclusion
It is evident from the present study that the ethyl acetate extract of K. pinnatum is rich in diterpene compounds and having potential antibacterial activity.
Keywords
Subject
Article metadata
| Title | Bioactivity of Diterpens from the Ethyl Acetate Extract of Kingiodendron pinnatum Rox. Hams |
|---|---|
| Authors | Komal Kumar Javarappa; Attemode Girijanna Devi Prasad; AJ Mahadesh Prasad; Chetana Mane |
| Affiliations | Department of Clinical and Experimental Medicine, Experimental Haematology Unit, University of Linkoping, Linkoping, Sweden; Department of Environmental Science; Department of Biochemistry, PBM Mahajana PG Centre, Mysore; Department of Biochemistry, University of Mysore, Karnataka, India |
| Corresponding author | kompaddy@yahoo.com |
| Journal | Pharmacognosy Research |
| Volume / Issue | Vol. 8, Issue 4 (2016) |
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