Media Standardization for Enhanced Production of Bacoside of Bacopa monnieri In situ Condition

Background: Secondary metabolites are known to not only play a major role in the adaptation of plants to their environment but also represent an important source of active pharmaceuticals Accumulation of such metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. The present study describes the enhancement of active principal bacoside of Bacopa monnieri by using stress and precursor compound. Materials and Methods: The study was done in situ condition. With incorporation of precursor compounds (phenylalanine and tyrosine [Ty]) and stress‑inducing compounds (ZnCl2, CoCl2, and CuSo4). Results: The maximum content of bacoside recorded in 7‑day‑old plant treated with 25 µM CoCl2 (5.313%) separately followed by 15‑day‑old plants treated with 60 mg/100 ml Ty (5.15%) and 15‑day‑old plants treated with 50 µM of CoCl2 (5.15%). Conclusion: Enhancement of bacoside in B. monnieri was observed more than 2% than in vivo condition by giving CoCl2 stress in situ condition. This kind of experiment is not well documented till date.