Open access · Peer reviewed · No APCs
Original ArticlePharmacognosy MagazineVol. 16 | Issue 05s | 2020 | pp. S540–S545Open access
DNA Barcoding Markers to Identify Intraspecies Genetic Variations in Three Ecotypes of Abrus precatorius L.
- 1,
- 2,
- 3*,
- 4,
- 5,
- 6
- 1 Research Officer (Biochemistry), Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India.
- 2 Survey of Medicinal Plants Unit, Central Research Institute for Unani Medicine, Hyderabad, Telangana, India.
- 3 Assistant Director In-charge, Regional Ayurveda Institute for Fundamental Research, India.
- 4 Research Officer (Pharmacognosy), Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India.
- 5 Assistant Director (Pharmacognosy), Central Council for Research in Ayurvedic Sciences, India.
- 6 Deputy Director General, Central Council for Research in Ayurvedic Sciences, New Delhi, India.
Published in Pharmacognosy Magazine
Correspondence: Goli Penchala Prasad
Assistant Director In-charge, Regional Ayurveda Institute for Fundamental Research, India.
Email: drpenchalaprasadgoli@gmail.com
Copyright: © 2020 Manuscript Technomedia. This is an open access article.
- Published:
- Nov 30, 2020
- Received:
- Feb 1, 2020
- Accepted:
- Jul 6, 2020
- DOI:
- 10.4103/pm.pm_23_20
How to cite
Marimuthu, S., Pratap, G. P., Prasad, G. P., Prasad, S. B., Mangal, A. K., & Srikanth, N. (2020). DNA Barcoding Markers to Identify Intraspecies Genetic Variations in Three Ecotypes of Abrus precatorius L. Pharmacognosy Magazine, 16(05s), S540–S545. https://doi.org/10.4103/pm.pm_23_20
Abstract
Objectives: The present study aims to compare three different varieties of A. precatorius which includes the species of white, black, and red which are used to study DNA barcoding marker and phylogenetic analysis. Materials and Methods: Whole genomic isolated DNA from three varieties (white, black, and red) of A. precatorius leaves and subjected to analysis of the polymerase chain reaction of rbcL, maturase K, and internal transcribed spacer 4,5 using 0.8% agarose gel electrophoresis. Results: The DNA barcoding markers and next‑generation sequencing can identify the intraspecies genetic variations among these closely related plant varieties of A. precatorius of white, black, and red. Conclusion: The intraspecies genetic variations among these three varieties of A. precatorius white, black, and red are closely related with A. precatorius isolate TMP 144 Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit gene 99.81%.
Keywords
Subject
Article metadata
| Title | DNA Barcoding Markers to Identify Intraspecies Genetic Variations in Three Ecotypes of Abrus precatorius L. |
|---|---|
| Authors | Srinivasan Marimuthu; G. P. Pratap; Goli Penchala Prasad; Shyam Baboo Prasad; Anupam Kumar Mangal; N. Srikanth |
| Affiliations | Research Officer (Biochemistry), Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India.; Survey of Medicinal Plants Unit, Central Research Institute for Unani Medicine, Hyderabad, Telangana, India.; Assistant Director In-charge, Regional Ayurveda Institute for Fundamental Research, India.; Research Officer (Pharmacognosy), Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India.; Assistant Director (Pharmacognosy), Central Council for Research in Ayurvedic Sciences, India.; Deputy Director General, Central Council for Research in Ayurvedic Sciences, New Delhi, India. |
| Corresponding author | drpenchalaprasadgoli@gmail.com |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 16, Issue 05s (2020) |
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