Original ArticlePharmacognosy MagazineVol. 16 | Issue 69 | 2020 | pp. 416–421Open access
Antiprotease Activity of Indigenous Medicinal Plants against Pakistani Echis carinatus Venom
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- 1 Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi.
- 2 Department of Pharmacy, LMDC, University of Health Sciences, Lahore.
- 3 Department of Chemistry, Abbottabad University of Science and Technology, Abbottabad.
- 4 Department of Zoology, Division of Science and Technology, University of Education, Multan Campus.
- 5 Institute of Food Sciences, Bahauddin Zakariya University.
- 6 Faculty of Pharmacy, Bahauddin Zakariya University, Multan.
- 7 Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Pakistan.
- 8 Department of Biology, 421 N, Woodland Blvd, Stetson University, DeLand, Florida, United States.
- 9 Department of Genetics, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
Published in Pharmacognosy Magazine
Correspondence: Muhammad Hassham Hassan Bin Asad
Department of Biology, 421 N, Woodland Blvd, Stetson University, DeLand, Florida, United States.
Email: hasshamasad@yahoo.com
Copyright: © 2020 Manuscript Technomedia. This is an open access article.
- Published:
- Jun 15, 2020
- Received:
- Aug 10, 2019
- Accepted:
- Jan 9, 2020
- DOI:
- 10.4103/pm.pm_355_19
How to cite
Aslam, N., Javed, T., Khalid, S., Shaheen, N., Fatima, S., Latif, M., Afzal, K., Afzal, S., Abbas, K., Yameen, M. A., Al-Kharaman, Y. M. S. A., Amirzada, M. I., McCleary, R. J. R., Rizvanov, A. A., & Asad, M. H. H. B. (2020). Antiprotease Activity of Indigenous Medicinal Plants against Pakistani Echis carinatus Venom. Pharmacognosy Magazine, 16(69), 416–421. https://doi.org/10.4103/pm.pm_355_19
Abstract
Objectives: This study was conducted to investigate the inhibitory potential of extracts from Pakistani medicinal plants against protease enzyme abundant in Echis carinatus venom. Materials and Methods: Organic extracts from different indigenous plant species and parts were used for in vitro determination of their inhibition against protease activity in snake venom. Active methanolic extracts were further fractioned using different solvents, and these fractions were also tested for antiprotease activity. Results: Results of this study show that Calotropis procera (Wild.) R. Br., Matthiola incana (L.) R. Br., and Terminalia arjuna Wight and Arn were able to neutralize the protease enzyme by 63%, 71%, and 66%, respectively. Trichodesma indicum (L.) R. Br. showed 51% inhibition of protease activity. Conclusion: The present study indicated about C. procera (Wild.) R. Br., M. incana (L.) R. Br., and T. arjuna Wight and Arn possessed inhibitor (s) against protease enzyme present in E. carinatus venom and would be worthwhile for development as treatment against envenomation in future.
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Article metadata
| Title | Antiprotease Activity of Indigenous Medicinal Plants against Pakistani Echis carinatus Venom |
|---|---|
| Authors | Nazia Aslam; Tariq Javed; Sofia Khalid; Nusrat Shaheen; Syeda Fatima; Muhammad Latif; Khurram Afzal; Samina Afzal; Khizar Abbas; Muhammad Arfat Yameen; Yasser M. S. A. Al-Kharaman; Muhammad Imran Amirzada; Ryan J. R. McCleary; Albert A. Rizvanov; Muhammad Hassham Hassan Bin Asad |
| Affiliations | Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi.; Department of Pharmacy, LMDC, University of Health Sciences, Lahore.; Department of Chemistry, Abbottabad University of Science and Technology, Abbottabad.; Department of Zoology, Division of Science and Technology, University of Education, Multan Campus.; Institute of Food Sciences, Bahauddin Zakariya University.; Faculty of Pharmacy, Bahauddin Zakariya University, Multan.; Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Pakistan.; Department of Biology, 421 N, Woodland Blvd, Stetson University, DeLand, Florida, United States.; Department of Genetics, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia. |
| Corresponding author | hasshamasad@yahoo.com |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 16, Issue 69 (2020) |
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