Original ArticlePharmacognosy MagazineVol. 16 | Issue 71 | 2020 | pp. 620–624Open access
Naringenin Reduced Migration in Osteosarcoma Cells through Downregulation of Matrix Metalloproteinase‑2 and Matrix Metalloproteinase‑9 and Snail
- 2,3*,
- 4,5*,
- 6,
- 3,
- 3,
- 7,8,9,
- 10,
- 3,11*,
- 12*
- 1 Department of Neurology, Division of Internal Medicine, Hsinchu Mackay Memorial Hospital, Hsinchu, Taiwan.
- 2 Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taiwan.
- 3 Department of Internal Medicine, Chung Shan Medical University Hospital, Taiwan.
- 4 School of Medicine, Chung Shan Medical University, Taiwan.
- 5 Department of Medical Research, Taichung Veterans General Hospital, Taiwan.
- 6 Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taiwan.
- 7 Department of Health and Nutrition Biotechnology, Asia University, Taiwan.
- 8 Chinese Medicine Research Center, China Medical University, Taiwan.
- 9 Department of Parasitology, School of Medicine, Chung Shan Medical University, Taiwan.
- 10 Clinical Laboratory, Chung Shan Medical University Hospital, Taiwan.
- 11 Division of Functional Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taiwan.
- 12 Department of Neurosurgery, Lin Shin Medical Corporation Lin Shin Hospital, Taichung, Taiwan.
Published in Pharmacognosy Magazine
Correspondence: Yuh‑Ming Chang
Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taiwan.; Department of Internal Medicine, Chung Shan Medical University Hospital, Taiwan.
Email: Ishsu405@yahoo.com.tw
Correspondence: Ming‑Cheng Lin
School of Medicine, Chung Shan Medical University, Taiwan.; Department of Medical Research, Taichung Veterans General Hospital, Taiwan.
Email: Ishsu405@yahoo.com.tw
Correspondence: Li‑Sung Hsu
Department of Internal Medicine, Chung Shan Medical University Hospital, Taiwan.; Division of Functional Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taiwan.
Email: Ishsu405@yahoo.com.tw
Correspondence: Ting‑Hsien Kao
Department of Neurosurgery, Lin Shin Medical Corporation Lin Shin Hospital, Taichung, Taiwan.
Email: joshuakth@gmail.com
Copyright: © 2020 Manuscript Technomedia. This is an open access article.
- Published:
- Oct 20, 2020
- Received:
- Jan 19, 2020
- Accepted:
- May 6, 2020
- DOI:
- 10.4103/pm.pm_31_20
How to cite
Chang, Y., Lin, M., Hong, L., Chen, P., Tsai, Y., Hseu, Y., Chen, K., Hsu, L., & Kao, T. (2020). Naringenin Reduced Migration in Osteosarcoma Cells through Downregulation of Matrix Metalloproteinase‑2 and Matrix Metalloproteinase‑9 and Snail. Pharmacognosy Magazine, 16(71), 620–624. https://doi.org/10.4103/pm.pm_31_20
Abstract
Objectives: In this study, we investigate the effects of naringenin on osteosarcoma. Materials and Methods: 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetra zolium bromide (MTT) assay was performed to detect the cell viability. Zymography assay and transwell assay were used to measure the matrix metalloproteinase (MMP) activity and migration ability. Western blot analysis was used to determine the expression of migration‑related proteins. Results: No overt alternation of cytotoxicity in response to different concentrations of naringenin for 24 h was found by MTT assay. MMP‑2 and MMP‑9 activities and expression were significantly repressed by naringenin in a dose‑dependent manner, as evidenced by zymography and Western blot analysis. Naringenin dose dependently reduced the expression of mesenchymal marker Snail. Conclusion: These results indicate that naringenin exhibited antimigration property through inhibition of MMP‑2 and MMP‑9 and downregulation of Snail expression. Thus, naringenin may be a potential inhibitor of metastasis of osteosarcoma.
Keywords
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Article metadata
| Title | Naringenin Reduced Migration in Osteosarcoma Cells through Downregulation of Matrix Metalloproteinase‑2 and Matrix Metalloproteinase‑9 and Snail |
|---|---|
| Authors | Yuh‑Ming Chang; Ming‑Cheng Lin; Ling‑Zong Hong; Pei‑Ni Chen; Yu ‑Hsin Tsai; You‑Cheng Hseu; Ke‑Min Chen; Li‑Sung Hsu; Ting‑Hsien Kao |
| Affiliations | Department of Neurology, Division of Internal Medicine, Hsinchu Mackay Memorial Hospital, Hsinchu, Taiwan.; Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taiwan.; Department of Internal Medicine, Chung Shan Medical University Hospital, Taiwan.; School of Medicine, Chung Shan Medical University, Taiwan.; Department of Medical Research, Taichung Veterans General Hospital, Taiwan.; Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taiwan.; Department of Health and Nutrition Biotechnology, Asia University, Taiwan.; Chinese Medicine Research Center, China Medical University, Taiwan.; Department of Parasitology, School of Medicine, Chung Shan Medical University, Taiwan.; Clinical Laboratory, Chung Shan Medical University Hospital, Taiwan.; Division of Functional Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taiwan.; Department of Neurosurgery, Lin Shin Medical Corporation Lin Shin Hospital, Taichung, Taiwan. |
| Corresponding author | Ishsu405@yahoo.com.tw |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 16, Issue 71 (2020) |
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