Content Determination and Anti-fatigue Effect of the Purified Anthocyanin from Purple Daucus carota

Background: The genus <em>Cissus</em> (Vitaceae) is known for its medicinal properties. <em>Cissus quadrangularis</em> is a well-known medicinal plant used in traditional medicine for bone healing. Objective: The present study was aimed to evaluate the anti-inflammatory and analgesic activities of the methanolic extract of <em>Cissus quadrangularis</em> (MECQ) in experimental animal models. Materials and Methods: The anti-inflammatory activity was evaluated using carrageenan-induced rat paw edema model, and analgesic activity was evaluated using acetic acid-induced writhing and tail-flick models in mice. Results: MECQ at doses of 200 and 400 mg/kg showed significant (P < 0.01) anti-inflammatory activity in carrageenan-induced rat paw edema. In analgesic models, MECQ showed significant (P < 0.01) reduction in the number of writhes in acetic acid-induced writhing model and increased the reaction time in tail-flick model. Conclusion: The results suggest that MECQ possesses significant anti-inflammatory and analgesic activities, which supports its traditional use.