Open access · Peer reviewed · No APCs
Original ArticlePharmacognosy MagazineVol. 17 | Issue 75 | 2021 | pp. 518–524Open access
Anti‑Inflammatory Effects of Malus toringoides Extract in Lipopolysaccharide‑Induced Human Umbilical Vein Endothelial Cells
- 2,
- 3,4,
- 2,
- 3,
- 3,
- 2,
- 2*
- 1 Department of Pharmacy, Key Laboratory of Pharmaceutical Research for Metabolic Diseases, Qingdao University of Science and Technology, Qingdao.
- 2 Department of Medicament, College of Medicine, Tibet University, Lhasa, China.
- 3 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.
- 4 Center of Tibetan Studies (Everest Research Institute), Tibet University, Lhasa, China.
Published in Pharmacognosy Magazine
Correspondence: Shan Huang
Department of Medicament, College of Medicine, Tibet University, Lhasa, China.
Email: huangshan@qust.edu.cn
Copyright: © 2021 Manuscript Technomedia. This is an open access article.
- Published:
- Nov 11, 2021
- Received:
- Aug 16, 2020
- Accepted:
- May 13, 2021
- DOI:
- 10.4103/pm.pm_357_20
How to cite
Fan, C., Dongzhi, Z., Dong, L., Yuan, R., Wang, J., Li, B., & Huang, S. (2021). Anti‑Inflammatory Effects of Malus toringoides Extract in Lipopolysaccharide ‑Induced Human Umbilical Vein Endothelial Cells. Pharmacognosy Magazine, 17(75), 518–524. https://doi.org/10.4103/pm.pm_357_20
Abstract
Objectives: The aim of the study was to explore the anti‑inflammatory effects and cellular mechanisms of extracts of M. toringoides (CBTM‑E375) in lipopolysaccharide (LPS)‑induced human umbilical vein endothelial cells (HUVECs). Materials and Methods: HUVECs were exposed to LPS, and the level of proinflammatory mediators was measured by enzyme‑linked immunosorbent assay. Furthermore, the activation of heme oxygenase‑1 (HO‑1), nuclear factor erythroid 2‑related factor 2 (Nrf2), and mitogen‑activated protein kinase (MAPK) was examined by Western blot and immunofluorescence analysis. Results: CBTM‑E375 significantly downregulated the levels of inflammatory mediators and upregulated the expression of HO‑1 by modulating Nrf2 translocation in HUVECs. The transfection of HO‑1 small interfering RNA into HUVECs actively reversed the effects of CBTM‑E375 in suppressing the expression of proinflammatory cytokines. Furthermore, MAPK activation in response to LPS was also blocked by CBTM‑E375. Conclusion: CBTM‑E375 exerts anti‑inflammatory effects, possibly by modulating the translocation of Nrf2 and expression of HO‑1, and inhibiting the phosphorylation of MAPK signaling pathway.
Keywords
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Article metadata
| Title | Anti‑Inflammatory Effects of Malus toringoides Extract in Lipopolysaccharide‑Induced Human Umbilical Vein Endothelial Cells |
|---|---|
| Authors | Chengde Fan; Zhuoma Dongzhi; Linsha Dong; Ruiying Yuan; Jule Wang; Bin Li; Shan Huang |
| Affiliations | Department of Pharmacy, Key Laboratory of Pharmaceutical Research for Metabolic Diseases, Qingdao University of Science and Technology, Qingdao.; Department of Medicament, College of Medicine, Tibet University, Lhasa, China.; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.; Center of Tibetan Studies (Everest Research Institute), Tibet University, Lhasa, China. |
| Corresponding author | huangshan@qust.edu.cn |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 17, Issue 75 (2021) |
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