Phytochemical Investigation of Bauhinia winitii Based on Alpha‑Glucosidase Inhibitory Effect and Molecular Docking Affirmation

Objectives: The aim of this study was to investigate the phytochemical constituents and anti‑alpha glucosidase activity of BW. Materials and Methods: Phytochemical study was based on the alpha‑glucosidase inhibitory effect. Isolated compounds were determined by chromatographic and spectroscopic techniques. The alpha‑glucosidase enzymatic inhibitory test was then connected with molecular docking affirmation. Results: The ethyl acetate and ethanol extracts from leaves and woods exhibited the strongest activity with above 60% inhibition. Six compounds were isolated from these extracts, naringenin (1), luteolin (2), isoquercitrin (3), griffonilide (4), lithospermoside (5), and epi‑β amyrin (6). The alpha‑glucosidase inhibitory test showed that flavonoids performed potential results with naringenin as the highest IC50 on 0.41 mM. The docking ensured the flavonoids activated at the same binding of alpha‑glucosidase enzyme active site. Conclusion: This study would be the first report of chemical constituents from BW with its isolated compounds was presented anti‑diabetic activity as alpha‑glucosidase inhibitors.