Research ArticlePharmacognosy MagazineVol. 5 | Issue 18 | 2009 | pp. 145–150Open access
In Vitro Propagation of Desmodium gangeticum (L.) DC. from Cotyledonary Nodal Explants
- 1,
- 1*,
- 1
- 1 Regional Research Institute (Ay.), Kothrud, Pune - 4110 38 (MH), India.
Published in Pharmacognosy Magazine
Correspondence: A.M Gurav
Regional Research Institute (Ay.), Kothrud, Pune - 4110 38 (MH), India.
Email: gurav_am@yahoo.co.in
Copyright: © 2009 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2009
- Received:
- Dec 9, 2008
- Accepted:
- Feb 5, 2009
How to cite
Vishwakarma, U., Gurav, A., & Sharma, P. (2009). In Vitro Propagation of Desmodium gangeticum (L.) DC. from Cotyledonary Nodal Explants. Pharmacognosy Magazine, 5(18), 145–150.
Abstract
An in vitro procedure for rapid multiplication of medicinally important plant Desmodium gangeticum (L.) DC. (Fabaceae), has been developed using cotyledonary nodal explant. An average of 9.2 shoots per explant were obtained by culturing cotyledonary nodal explant on Murashige and Skoog’s medium containing 8.8 µM BAP and 21.2 µM NAA, in combination, within 28 days. These shoots were rooted on half strength MS medium supplemented with IAA 17.1 µM. Rooted plantlets were hardened using 1:1:1 mixture of soil, river sand and vermiculite under green house conditions.
Keywords
Subject
Article metadata
| Title | In Vitro Propagation of Desmodium gangeticum (L.) DC. from Cotyledonary Nodal Explants |
|---|---|
| Authors | U.R. Vishwakarma; A.M Gurav; P.C. Sharma |
| Affiliations | Regional Research Institute (Ay.), Kothrud, Pune - 4110 38 (MH), India. |
| Corresponding author | gurav_am@yahoo.co.in |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 5, Issue 18 (2009) |
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